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Reactive oxygen species regulate DNA copy number in isolated yeast mitochondria by triggering recombination-mediated replication
https://sucra.repo.nii.ac.jp/records/13390
https://sucra.repo.nii.ac.jp/records/13390079e1e45-fffa-40af-91aa-61a0fbe24a46
名前 / ファイル | ライセンス | アクション |
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A1002839.pdf (3.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-05-11 | |||||
タイトル | ||||||
タイトル | Reactive oxygen species regulate DNA copy number in isolated yeast mitochondria by triggering recombination-mediated replication | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hori, Akiko
× Hori, Akiko× 吉田, 稔× Shibata, Takehiko× Ling, Feng |
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著者 ローマ字 | ||||||
Hori, Akiko | ||||||
著者 ローマ字 | ||||||
Yoshida, Minoru | ||||||
著者 ローマ字 | ||||||
Shibata, Takehiko | ||||||
著者 ローマ字 | ||||||
Ling, Feng | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科 | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
Graduate School of Science and Engineering, Saitama University | ||||||
書誌情報 |
NUCLEIC ACIDS RESEARCH 巻 37, 号 3, p. 749-761, 発行日 2009 |
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年月次 | ||||||
2009-2 | ||||||
出版者名 | ||||||
出版者 | Oxford University Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03051048 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1093/nar/gkn993 | |||||
関連サイト | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://nar.oxfordjournals.org/cgi/content/abstract/37/3/749 | http://nar.oxfordjournals.org/cgi/content/abstract/37/3/749 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Mitochondrial DNA (mtDNA) encodes proteins that are essential for cellular ATP production. Reactive oxygen species (ROS) are respiratory byproducts that damage mtDNA and other cellular components. In Saccharomyces cerevisiae, the oxidized base excision-repair enzyme Ntg1 introduces a double-stranded break (DSB) at the mtDNA replication origin ori5; this DSB initiates the rolling-circle mtDNA replication mediated by the homologous DNA pairing protein Mhr1. Thus, ROS may play a role in the regulation of mtDNA copy number. Here, we show that the treatment of isolated mitochondria with low concentrations of hydrogen peroxide increased mtDNA copy number in an Ntg1- and Mhr1-dependent manner. This treatment elevated the DSB levels at ori5 of hypersuppressive [rho(-)] mtDNA only if Ntg1 was active. In vitro Ntg1-treatment of hypersuppressive [rho(-)] mtDNA extracted from hydrogen peroxide-treated mitochondria revealed increased oxidative modifications at ori5 loci. We also observed that purified Ntg1 created breaks in single-stranded DNA harboring oxidized bases, and that ori5 loci have single-stranded character. Furthermore, chronic low levels of hydrogen peroxide increased in vivo mtDNA copy number. We therefore propose that ROS act as a regulator of mtDNA copy number, acting through the Mhr1-dependent initiation of rolling-circle replication promoted by Ntg1-induced DSB in the single-stranded regions at ori5. | |||||
版 | ||||||
[著者版] | ||||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2009-05-11 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1002839 |