WEKO3
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酵素を用いたオリゴヌクレオチドの合成と応用<ミニノート>
https://doi.org/10.24561/00014759
https://doi.org/10.24561/00014759d77d409c-b255-4562-ada2-597113a15195
名前 / ファイル | ライセンス | アクション |
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KY-AN10402595-11-06.pdf (167.2 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2009-11-12 | |||||
タイトル | ||||||
タイトル | 酵素を用いたオリゴヌクレオチドの合成と応用<ミニノート> | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.24561/00014759 | |||||
ID登録タイプ | JaLC | |||||
タイトル(別言語) | ||||||
その他のタイトル | Enzymatic Synthesis of Oligonucleotides and Their Application | |||||
著者 |
木下, 保則
× 木下, 保則 |
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著者 ローマ字 | ||||||
KINOSHITA, Yasunori | ||||||
著者 所属 | ||||||
埼玉大学工学部環境化学工学科 | ||||||
著者 所属(別言語) | ||||||
Department of Environmental Chemistry, Faculty of Engineering., Saitama University | ||||||
書誌情報 |
CACS FORUM 巻 11, p. 25-27, 発行日 1991 |
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年月次 | ||||||
1991-11 | ||||||
出版者名 | ||||||
出版者 | 埼玉大学分析センター | |||||
概要 | ||||||
内容記述タイプ | Other | |||||
内容記述 | A new rapid method to synthesize an oligonucleotide 9-mer of any base sequence is proposed. According to the base sequence designed for the oligomer, a 5'tetramer and a 3'tetramer are picked up from a library of tetradeoxyribonucleotides, which have been previously prepared using a DNA synthesizer. The 5'tetramer is added with a mono-ribo-nucleotide (according to the 5th base of the desired sequence) using the terminal deoxynucleotidyl transferase and the 3'tetramer is phosphorylated at 5'terminus using the T4 polynucleotide kinase. Then, the 5'pentamer and the 3'tetramer are combined with the T4 RNA ligase, resulting in a 9-mer having the desired sequence. The 9-mer is proved to serve as a primer for a DNA polymerase reaction without purification steps. This procedure which requires only 2h, can rapidly provide primers for some method of the large-scale DNAsequencmg. | |||||
版 | ||||||
[出版社版] | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2010-01-14 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
KY-AN10402595-11-06 |