WEKO3
アイテム
{"_buckets": {"deposit": "11aba317-1480-4e11-abe0-682ae762a7c4"}, "_deposit": {"created_by": 3, "id": "18558", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "18558"}, "status": "published"}, "_oai": {"id": "oai:sucra.repo.nii.ac.jp:00018558", "sets": ["926"]}, "author_link": ["29547", "18138"], "item_120_alternative_title_1": {"attribute_name": "タイトル(別言語)", "attribute_value_mlt": [{"subitem_alternative_title": "Aquatic Fauna of Hozoji Pond Surveyed Using Environmental DNA Analyses : Compared to Field Surveys"}]}, "item_120_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2019", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "331", "bibliographicPageStart": "317", "bibliographicVolumeNumber": "68", "bibliographic_titles": [{"bibliographic_title": "埼玉大学紀要. 教育学部"}, {"bibliographic_title": "Journal of Saitama University. Faculty of Education", "bibliographic_titleLang": "en"}]}]}, "item_120_date_31": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2019-03-05", "subitem_date_issued_type": "Created"}]}, "item_120_description_19": {"attribute_name": "概要", "attribute_value_mlt": [{"subitem_description": "Hozoji pond is a natural habitat of the almost extinct aquatic carnivorous plant, Aldrovanda vesiculosa L., where aquatic fauna has been investigated using fishing implements since 2010. Recent our field surveys using the fishing implements have shown small-sized fish species exist in the pond in addition to a large number of shrimps, tadpoles, and crayfishes; signs of large fish have been just witnessed there. Here we attempted the identification of aquatic vertebrates using environmental DNA (eDNA) and compared the results to those of field surveys in Hozoji pond and the surrounding, Mitagaya pond. We performed eDNA experiments using newly designed primers, cloning an eDNA fragment, and digesting with restriction enzymes. The results showed 49% of 70 clones were positively identified as vertebrates. Besides, gibels Carassinus sp. and snakehead Channa argus were detected, which have not captured these days. The species of 8 individuals of Carassinus sp. identified from mitochondrial DNA sequencing were distinct from those on morphological traits, suggesting crossing occurs in the pond. The methods of eDNA analysis in this study are competent to reveal a closed aquatic fauna, like Hozoji pond. The detailed fauna could be understood using eDNA as the complement to field surveys.", "subitem_description_type": "Other"}]}, "item_120_description_29": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "text", "subitem_description_type": "Other"}]}, "item_120_description_30": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_120_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24561/00018528", "subitem_identifier_reg_type": "JaLC"}]}, "item_120_publisher_11": {"attribute_name": "出版者名", "attribute_value_mlt": [{"subitem_publisher": "埼玉大学教育学部"}]}, "item_120_publisher_12": {"attribute_name": "出版者名(別言語)", "attribute_value_mlt": [{"subitem_publisher": "Faculty of Education, Saitama University"}]}, "item_120_source_id_14": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "18815146", "subitem_source_identifier_type": "ISSN"}]}, "item_120_text_27": {"attribute_name": "版", "attribute_value_mlt": [{"subitem_text_value": "[出版社版]"}]}, "item_120_text_3": {"attribute_name": "著者 ローマ字", "attribute_value_mlt": [{"subitem_text_value": "YOSHIDA, Tatsuya"}, {"subitem_text_value": "HIBINO, Taku"}]}, "item_120_text_32": {"attribute_name": "アイテムID", "attribute_value_mlt": [{"subitem_text_value": "KY-AA12318206-6801-23"}]}, "item_120_text_4": {"attribute_name": "著者 所属", "attribute_value_mlt": [{"subitem_text_value": "埼玉大学大学院教育学研究科自然科学専修"}, {"subitem_text_value": "埼玉大学教育学部自然科学講座"}]}, "item_120_text_5": {"attribute_name": "著者 所属(別言語)", "attribute_value_mlt": [{"subitem_text_value": "Graduate School of Education, Saitama University"}, {"subitem_text_value": "Faculty of Education, Saitama University"}]}, "item_120_text_9": {"attribute_name": "年月次", "attribute_value_mlt": [{"subitem_text_value": "2019"}]}, "item_120_version_type_28": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "吉田, 竜矢"}, {"creatorName": "ヨシダ, タツヤ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "29547", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "日比野, 拓"}, {"creatorName": "ヒビノ, タク", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "18138", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "sNhSFxBx", "nameIdentifierScheme": "研究者総覧", "nameIdentifierURI": "http://s-read.saitama-u.ac.jp/researchers/pages/researcher/sNhSFxBx"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-03-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KY-AA12318206-6801-23.pdf", "filesize": [{"value": "2.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2800000.0, "url": {"label": "KY-AA12318206-6801-23.pdf", "url": "https://sucra.repo.nii.ac.jp/record/18558/files/KY-AA12318206-6801-23.pdf"}, "version_id": "07090ea2-4a15-479b-81ef-ef8da7fc4f66"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "eDNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "種多様性", "subitem_subject_scheme": "Other"}, {"subitem_subject": "宝蔵寺沼", "subitem_subject_scheme": "Other"}, {"subitem_subject": "特別天然記念物", "subitem_subject_scheme": "Other"}, {"subitem_subject": "水生食虫植物", "subitem_subject_scheme": "Other"}, {"subitem_subject": "field survey", "subitem_subject_scheme": "Other"}, {"subitem_subject": "crossing", "subitem_subject_scheme": "Other"}, {"subitem_subject": "species diversity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "special natural monument", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "環境DNA分析を用いた宝蔵寺沼水生動物の同定 : 野外調査との比較\u003c数学・自然科学\u003e", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "環境DNA分析を用いた宝蔵寺沼水生動物の同定 : 野外調査との比較\u003c数学・自然科学\u003e"}]}, "item_type_id": "120", "owner": "3", "path": ["926"], "permalink_uri": "https://doi.org/10.24561/00018528", "pubdate": {"attribute_name": "公開日", "attribute_value": "2019-03-05"}, "publish_date": "2019-03-05", "publish_status": "0", "recid": "18558", "relation": {}, "relation_version_is_last": true, "title": ["環境DNA分析を用いた宝蔵寺沼水生動物の同定 : 野外調査との比較\u003c数学・自然科学\u003e"], "weko_shared_id": 3}
環境DNA分析を用いた宝蔵寺沼水生動物の同定 : 野外調査との比較<数学・自然科学>
https://doi.org/10.24561/00018528
https://doi.org/10.24561/00018528f6cfae20-a9f7-45a0-a8fc-c15d84b7833a
名前 / ファイル | ライセンス | アクション |
---|---|---|
KY-AA12318206-6801-23.pdf (2.8 MB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2019-03-05 | |||||
タイトル | ||||||
タイトル | 環境DNA分析を用いた宝蔵寺沼水生動物の同定 : 野外調査との比較<数学・自然科学> | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | eDNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 種多様性 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 宝蔵寺沼 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 特別天然記念物 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 水生食虫植物 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | field survey | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | crossing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | species diversity | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | special natural monument | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.24561/00018528 | |||||
ID登録タイプ | JaLC | |||||
タイトル(別言語) | ||||||
その他のタイトル | Aquatic Fauna of Hozoji Pond Surveyed Using Environmental DNA Analyses : Compared to Field Surveys | |||||
著者 |
吉田, 竜矢
× 吉田, 竜矢× 日比野, 拓 |
|||||
著者 ローマ字 | ||||||
YOSHIDA, Tatsuya | ||||||
著者 ローマ字 | ||||||
HIBINO, Taku | ||||||
著者 所属 | ||||||
埼玉大学大学院教育学研究科自然科学専修 | ||||||
著者 所属 | ||||||
埼玉大学教育学部自然科学講座 | ||||||
著者 所属(別言語) | ||||||
Graduate School of Education, Saitama University | ||||||
著者 所属(別言語) | ||||||
Faculty of Education, Saitama University | ||||||
書誌情報 |
埼玉大学紀要. 教育学部 en : Journal of Saitama University. Faculty of Education 巻 68, 号 1, p. 317-331, 発行日 2019 |
|||||
年月次 | ||||||
2019 | ||||||
出版者名 | ||||||
出版者 | 埼玉大学教育学部 | |||||
出版者名(別言語) | ||||||
出版者 | Faculty of Education, Saitama University | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18815146 | |||||
概要 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Hozoji pond is a natural habitat of the almost extinct aquatic carnivorous plant, Aldrovanda vesiculosa L., where aquatic fauna has been investigated using fishing implements since 2010. Recent our field surveys using the fishing implements have shown small-sized fish species exist in the pond in addition to a large number of shrimps, tadpoles, and crayfishes; signs of large fish have been just witnessed there. Here we attempted the identification of aquatic vertebrates using environmental DNA (eDNA) and compared the results to those of field surveys in Hozoji pond and the surrounding, Mitagaya pond. We performed eDNA experiments using newly designed primers, cloning an eDNA fragment, and digesting with restriction enzymes. The results showed 49% of 70 clones were positively identified as vertebrates. Besides, gibels Carassinus sp. and snakehead Channa argus were detected, which have not captured these days. The species of 8 individuals of Carassinus sp. identified from mitochondrial DNA sequencing were distinct from those on morphological traits, suggesting crossing occurs in the pond. The methods of eDNA analysis in this study are competent to reveal a closed aquatic fauna, like Hozoji pond. The detailed fauna could be understood using eDNA as the complement to field surveys. | |||||
版 | ||||||
[出版社版] | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2019-03-05 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
KY-AA12318206-6801-23 |