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In the current study, we determined the cDNA and amino acid sequences of motilin and its receptor (GPR38) in Japanese quail, and studied the distribution of motilin-producing cells and the expression of GPR38 mRNA in the quail GI tract. Using an in vitro organ bath, we examined the motilin-induced contractile properties of different regions of the quail GI tract. We also determined the age-dependent changes and mechanisms of motilin-induced contraction in the proventriculus and duodenum of the quail. Mature quail motilin was composed of 22 amino acids, and showed high homology to chicken (95.4%), human (72.7%), and dog (72.7%) motilin. Immunohistochemical analysis revealed that motilin-immunopositive cells were present in the mucosal layer of the duodenum (23.4 ± 4.6 cells/mm2), jejunum (15.2 ± 0.8 cells/mm2), and ileum (2.5 ± 0.7 cells/mm2), but were not observed in the crop, proventriculus, and colon. We also cloned quail GPR38-encoding cDNA from the medulla oblongata and obtained a partial sequence of 183 bp. Real-time PCR analysis showed that quail GPR38 mRNA was expressed in the crop, proventriculus, duodenum, jejunum, ileum, colon, liver, heart, and muscle. In the organ bath study, chicken motilin evoked dose-dependent contraction of the proventriculus, duodenum, jejunum, and ileum. In contrast, chicken ghrelin had no effect on contraction in the quail GI tract. Motilin-induced contraction in the duodenum was not inhibited by atropine, hexamethonium, ritanserin, ondansetron, GR125487, or tetrodotoxin. However, motilin-induced contractions in the proventriculus were significantly inhibited by atropine and tetrodotoxin. In addition, motilin-induced contraction was significantly decreased with age in the proventriculus, but not in the duodenum. These results suggest that motilin stimulates gastrointestinal motility, and that the site of action for motilin is different between the small intestine and proventriculus of Japanese quail.", "subitem_description_type": "Abstract"}]}, "item_113_description_24": {"attribute_name": "目次", "attribute_value_mlt": [{"subitem_description": "ABSTRACT I\nTABLE OF CONTENTS III\nLIST OF FIGURES VI\nLIST OF APPENDICES VIII\nChapter 1: General introduction and objectives 1\n1.1. Research background 1\n1.1.1. The migrating motor complex of gastrointestinal (GI) motility 1\n1.1.2. Motilin 2\n1.1.3. Ghrelin 3\n1.1.4. Co-ordinated action of motilin and ghrelin in the GI tract 5\n1.1.5. Regulatory mechanism of motilin-induced GI contraction 5\n1.1.6. Japanese quail (Coturnix japonica) 6\n1.2. Purpose of the study 8\nChapter 2: Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in Japanese quail 9\n2.1. Introduction 9\n2.2. Materials and Methods 12\n2.2.1. Drugs used 12\n2.2.2. Animals 12\n2.2.3. Cloning of quail motilin cDNA 12\n2.2.4. Tissue preparation 13\n2.2.4.1. Immunohistochemistry 14\n2.2.4.2. Morphometric analysis 15\n2.2.5. Organ bath study 15\n2.2.6. Statistical analysis 17\n2.3. Results 18\n2.3.1. Cloning of quail motilin cDNA 18\n2.3.2. Localization of motilin immunopositive (motilin-ip) cells in the GI tract of quail 18\n2.3.3. Effects of motilin on the GI tract of quail 19\n2.3.4. Effects of ghrelin on the GI tract of quail 19\n2.3.5. Effects of co-administration of motilin and ghrelin on the GI tract of quail 20\n2.3.6. Mechanism of chicken motilin-induced contraction in the duodenum of quail 20\n2.3.7. Mechanism of chicken motilin-induced contraction in the proventriculus of quail 20\n2.4. Discussion 22\n2.4.1. cDNA and amino acid sequences of quail motilin 22\n2.4.2. Immunohistochemical analysis of quail motilin 22\n2.4.3. Effects of motilin and ghrelin, alone or together, on gastrointestinal motility in Japanese quail 23\n2.4.4. Mechanism of motilin-induced contraction in quail proventriculus and duodenum 23\n2.4.5. Physiological significance of MMC in relation to motilin 25\n2.5. Summary 26\nChapter 3: Molecular identification and expression of GPR38 in the gastrointestinal tract of Japanese quail 27\n3.1. Introduction 27\n3.2. Materials and methods 30\n3.2.1. Animals 30\n3.2.2. Cloning of quail GPR38 30\n3.2.3. Reverse transcription (RT) and quantitative real-time PCR (Q-PCR) analysis 31\n3.2.4. Organ bath study 32\n3.2.5. Statistical analysis 33\n3.3. Results 34\n3.3.1. Cloning of quail GPR38 34\n3.3.2. Expression of GPR38 mRNA in quail tissues 34\n3.3.3. Age-dependent changes in motilin-induced contraction in the GI tract of Japanese quail 34\n3.4. Discussion 36\n3.5. Summary 39\n4.0. CONCLUSION 40\nABBREVIATIONS 41\nREFERENCES 43\nFIGURES 63\nAPPENDICES 87\nACKNOWLEDGEMENTS 92", "subitem_description_type": "Other"}]}, "item_113_description_25": {"attribute_name": "注記", "attribute_value_mlt": [{"subitem_description": "主指導教員 : 坂田一郎", "subitem_description_type": "Other"}]}, "item_113_description_33": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "text", "subitem_description_type": "Other"}]}, "item_113_description_34": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_113_dissertation_number_19": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第1024号"}]}, "item_113_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24561/00010414", "subitem_identifier_reg_type": "JaLC"}]}, "item_113_publisher_11": {"attribute_name": "出版者名", "attribute_value_mlt": [{"subitem_publisher": "埼玉大学大学院理工学研究科"}]}, "item_113_publisher_12": {"attribute_name": "出版者名(別言語)", "attribute_value_mlt": [{"subitem_publisher": "Graduate School of Science and Engineering, Saitama University"}]}, "item_113_record_name_8": {"attribute_name": "書誌", "attribute_value_mlt": [{"subitem_record_name": "博士論文(埼玉大学大学院理工学研究科(博士後期課程))"}]}, "item_113_text_31": {"attribute_name": "版", "attribute_value_mlt": [{"subitem_text_value": "[出版社版]"}]}, "item_113_text_36": {"attribute_name": "アイテムID", "attribute_value_mlt": [{"subitem_text_value": "GD0000832"}]}, "item_113_text_4": {"attribute_name": "著者 所属", "attribute_value_mlt": [{"subitem_text_value": "埼玉大学大学院理工学研究科(博士後期課程)理工学専攻"}]}, "item_113_text_5": {"attribute_name": "著者 所属(別言語)", "attribute_value_mlt": [{"subitem_text_value": "Graduate School of Science and Engineering, Saitama University"}]}, "item_113_version_type_32": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "AUVIJIT, SAHA APU", "creatorNameLang": "en"}, {"creatorName": "アビィジッ, サハ アプ", "creatorNameLang": "ja-Kana"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "GD0000832.pdf", "filesize": [{"value": "4.1 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 4099999.9999999995, "url": {"label": "GD0000832.pdf", "objectType": "fulltext", "url": "https://sucra.repo.nii.ac.jp/record/10420/files/GD0000832.pdf"}, "version_id": "eb9b5114-65f1-4946-91e8-22aaf78a44fb"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Regulatory mechanism of motilin-induced gastrointestinal motility in Japanese quail", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Regulatory mechanism of motilin-induced gastrointestinal motility in Japanese quail", "subitem_title_language": "en"}]}, "item_type_id": "113", "owner": "15", "path": ["509"], "permalink_uri": "https://doi.org/10.24561/00010414", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-07-19"}, "publish_date": "2017-07-19", "publish_status": "0", "recid": "10420", "relation": {}, "relation_version_is_last": true, "title": ["Regulatory mechanism of motilin-induced gastrointestinal motility in Japanese quail"], "weko_shared_id": -1}
Regulatory mechanism of motilin-induced gastrointestinal motility in Japanese quail
https://doi.org/10.24561/00010414
https://doi.org/10.24561/000104144fe931dc-63a5-4168-8938-55350b6acaf4
名前 / ファイル | ライセンス | アクション |
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GD0000832.pdf (4.1 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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公開日 | 2017-07-19 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Regulatory mechanism of motilin-induced gastrointestinal motility in Japanese quail | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
資源タイプ | doctoral thesis | |||||||||
ID登録 | ||||||||||
ID登録 | 10.24561/00010414 | |||||||||
ID登録タイプ | JaLC | |||||||||
アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
タイトル(別言語) | ||||||||||
その他のタイトル | ウズラにおけるモチリン誘導性消化管運動調節機構の研究 | |||||||||
著者 |
AUVIJIT, SAHA APU
× AUVIJIT, SAHA APU
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著者 所属 | ||||||||||
埼玉大学大学院理工学研究科(博士後期課程)理工学専攻 | ||||||||||
著者 所属(別言語) | ||||||||||
Graduate School of Science and Engineering, Saitama University | ||||||||||
書誌 | ||||||||||
収録物名 | 博士論文(埼玉大学大学院理工学研究科(博士後期課程)) | |||||||||
書誌情報 |
発行日 2016 |
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出版者名 | ||||||||||
出版者 | 埼玉大学大学院理工学研究科 | |||||||||
出版者名(別言語) | ||||||||||
出版者 | Graduate School of Science and Engineering, Saitama University | |||||||||
形態 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | viii, 93p | |||||||||
学位授与番号 | ||||||||||
学位授与番号 | 甲第1024号 | |||||||||
学位授与年月日 | ||||||||||
学位授与年月日 | 2016-09-23 | |||||||||
学位名 | ||||||||||
学位名 | 博士(学術) | |||||||||
学位授与機関 | ||||||||||
学位授与機関識別子Scheme | kakenhi | |||||||||
学位授与機関識別子 | 12401 | |||||||||
学位授与機関名 | 埼玉大学 | |||||||||
抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | Motilin is a peptide hormone that plays an important role in the regulation of gastrointestinal (GI) motility. In the current study, we determined the cDNA and amino acid sequences of motilin and its receptor (GPR38) in Japanese quail, and studied the distribution of motilin-producing cells and the expression of GPR38 mRNA in the quail GI tract. Using an in vitro organ bath, we examined the motilin-induced contractile properties of different regions of the quail GI tract. We also determined the age-dependent changes and mechanisms of motilin-induced contraction in the proventriculus and duodenum of the quail. Mature quail motilin was composed of 22 amino acids, and showed high homology to chicken (95.4%), human (72.7%), and dog (72.7%) motilin. Immunohistochemical analysis revealed that motilin-immunopositive cells were present in the mucosal layer of the duodenum (23.4 ± 4.6 cells/mm2), jejunum (15.2 ± 0.8 cells/mm2), and ileum (2.5 ± 0.7 cells/mm2), but were not observed in the crop, proventriculus, and colon. We also cloned quail GPR38-encoding cDNA from the medulla oblongata and obtained a partial sequence of 183 bp. Real-time PCR analysis showed that quail GPR38 mRNA was expressed in the crop, proventriculus, duodenum, jejunum, ileum, colon, liver, heart, and muscle. In the organ bath study, chicken motilin evoked dose-dependent contraction of the proventriculus, duodenum, jejunum, and ileum. In contrast, chicken ghrelin had no effect on contraction in the quail GI tract. Motilin-induced contraction in the duodenum was not inhibited by atropine, hexamethonium, ritanserin, ondansetron, GR125487, or tetrodotoxin. However, motilin-induced contractions in the proventriculus were significantly inhibited by atropine and tetrodotoxin. In addition, motilin-induced contraction was significantly decreased with age in the proventriculus, but not in the duodenum. These results suggest that motilin stimulates gastrointestinal motility, and that the site of action for motilin is different between the small intestine and proventriculus of Japanese quail. | |||||||||
目次 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | ABSTRACT I TABLE OF CONTENTS III LIST OF FIGURES VI LIST OF APPENDICES VIII Chapter 1: General introduction and objectives 1 1.1. Research background 1 1.1.1. The migrating motor complex of gastrointestinal (GI) motility 1 1.1.2. Motilin 2 1.1.3. Ghrelin 3 1.1.4. Co-ordinated action of motilin and ghrelin in the GI tract 5 1.1.5. Regulatory mechanism of motilin-induced GI contraction 5 1.1.6. Japanese quail (Coturnix japonica) 6 1.2. Purpose of the study 8 Chapter 2: Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in Japanese quail 9 2.1. Introduction 9 2.2. Materials and Methods 12 2.2.1. Drugs used 12 2.2.2. Animals 12 2.2.3. Cloning of quail motilin cDNA 12 2.2.4. Tissue preparation 13 2.2.4.1. Immunohistochemistry 14 2.2.4.2. Morphometric analysis 15 2.2.5. Organ bath study 15 2.2.6. Statistical analysis 17 2.3. Results 18 2.3.1. Cloning of quail motilin cDNA 18 2.3.2. Localization of motilin immunopositive (motilin-ip) cells in the GI tract of quail 18 2.3.3. Effects of motilin on the GI tract of quail 19 2.3.4. Effects of ghrelin on the GI tract of quail 19 2.3.5. Effects of co-administration of motilin and ghrelin on the GI tract of quail 20 2.3.6. Mechanism of chicken motilin-induced contraction in the duodenum of quail 20 2.3.7. Mechanism of chicken motilin-induced contraction in the proventriculus of quail 20 2.4. Discussion 22 2.4.1. cDNA and amino acid sequences of quail motilin 22 2.4.2. Immunohistochemical analysis of quail motilin 22 2.4.3. Effects of motilin and ghrelin, alone or together, on gastrointestinal motility in Japanese quail 23 2.4.4. Mechanism of motilin-induced contraction in quail proventriculus and duodenum 23 2.4.5. Physiological significance of MMC in relation to motilin 25 2.5. Summary 26 Chapter 3: Molecular identification and expression of GPR38 in the gastrointestinal tract of Japanese quail 27 3.1. Introduction 27 3.2. Materials and methods 30 3.2.1. Animals 30 3.2.2. Cloning of quail GPR38 30 3.2.3. Reverse transcription (RT) and quantitative real-time PCR (Q-PCR) analysis 31 3.2.4. Organ bath study 32 3.2.5. Statistical analysis 33 3.3. Results 34 3.3.1. Cloning of quail GPR38 34 3.3.2. Expression of GPR38 mRNA in quail tissues 34 3.3.3. Age-dependent changes in motilin-induced contraction in the GI tract of Japanese quail 34 3.4. Discussion 36 3.5. Summary 39 4.0. CONCLUSION 40 ABBREVIATIONS 41 REFERENCES 43 FIGURES 63 APPENDICES 87 ACKNOWLEDGEMENTS 92 |
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注記 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 主指導教員 : 坂田一郎 | |||||||||
版 | ||||||||||
[出版社版] | ||||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
資源タイプ | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | text | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
作成日 | ||||||||||
日付 | 2017-07-19 | |||||||||
日付タイプ | Created | |||||||||
アイテムID | ||||||||||
GD0000832 |