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Characterization of high-light-responsive promoters of the psaAB genes in Synechocystis sp PCC 6803
https://sucra.repo.nii.ac.jp/records/12517
https://sucra.repo.nii.ac.jp/records/1251786e6a697-1cc8-4775-859f-f74bcebea3a0
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
A1002032.pdf (726.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2007-03-08 | |||||
| タイトル | ||||||
| タイトル | Characterization of high-light-responsive promoters of the psaAB genes in Synechocystis sp PCC 6803 | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
日原, 由香子
× 日原, 由香子 |
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| 著者 ローマ字 | ||||||
| 値 | Hihara, Yukako | |||||
| 著者 ローマ字 | ||||||
| 値 | Muramatsu, Masayuki | |||||
| 著者 ローマ字 | ||||||
| 値 | Nakamura, Kinu | |||||
| 著者 ローマ字 | ||||||
| 値 | Sonoike, Kintake | |||||
| 著者 所属 | ||||||
| 値 | 埼玉大学大学院理工学研究科 | |||||
| 著者 所属(別言語) | ||||||
| 値 | Saitama University, Graduate School of Science and Engineering | |||||
| 書誌情報 |
Plant and cell physiology 巻 47, 号 7, p. 878-890, 発行日 2006 |
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| 年月次 | ||||||
| 値 | 2006-7 | |||||
| 出版者名 | ||||||
| 出版者 | Oxford Univ. Press | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1093/pcp/pcj060 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In cyanobacteria, transcription of genes encoding subunits of PSI is tightly repressed under high-light conditions. To elucidate the molecular mechanism, we examined the promoter architecture of the psaAB genes encoding reaction center subunits of PSI in a cyanobacterium Synechocystis sp. PCC 6803. Primer extension analysis showed the existence of two promoters, P1 and P2, both of which are responsible for the light-intensity dependent transcription of the psaAB genes. Deletion analysis of the upstream region of psaAB fused to bacterial luciferase reporter genes (luxAB) indicated that the light response of these promoters is achieved in totally different manner. The cis-element required for the light response of P1, designated as PE1, was located just upstream of the -35 element of P1 and was comprised of AT-rich sequence showing significant homology to UP-element often found in strong bacterial promoters. PE1 activated P1 under low-light conditions and the down-regulation of P1 was achieved by rapid inactivation of PE1 upon the shift to high-light conditions. On the other hand, the cis-element required for the light response of P2, designated as HNE2, was located upstream of P1 region, far from the basal promoter of P2. The down-regulation of P2 seemed to be attained through the negative regulation by HNE2 activated only under high-light conditions. DNA gel mobility shift assays showed that at least five regions in psaAB promoters were responsible for the binding of putative regulatory protein factors. | |||||
| 注記 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Plant and cell physiology following peer review. The definitive publisher-authenticated version Oxford University Press, Plant and Cell Physiology, 2006 47(7):878-890; doi:10.1093/pcp/pcj060 is available online at: http://pcp.oxfordjournals.org/cgi/content/abstract/47/7/878. | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 作成日 | ||||||
| 日付 | 2007-03-05 | |||||
| 日付タイプ | Created | |||||
| アイテムID | ||||||
| 値 | A1002032 | |||||
