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An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering
https://sucra.repo.nii.ac.jp/records/12945
https://sucra.repo.nii.ac.jp/records/1294576255265-7134-4413-b672-b2c4c45e2399
名前 / ファイル | ライセンス | アクション |
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A1002279.pdf (2.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-17 | |||||
タイトル | ||||||
タイトル | An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | In vitro selection | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mRNA-display | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | in vitro virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | C-terminus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Non-natural amino acid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | T4 RNA Ligase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
上野, 真吾
× 上野, 真吾× Arai, Hidenao× Suzuki, Miho× 伏見, 譲 |
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著者 ローマ字 | ||||||
Ueno, Shingo | ||||||
著者 ローマ字 | ||||||
Arai, Hidenao | ||||||
著者 ローマ字 | ||||||
Suzuki, Miho | ||||||
著者 ローマ字 | ||||||
Husimi, Yuzuru | ||||||
著者 所属 | ||||||
埼玉大学理学部細胞制御学研究室 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科物質科学部門物質機能領域 | ||||||
著者 所属(別言語) | ||||||
Faculty of Science, Saitama University | ||||||
著者 所属(別言語) | ||||||
Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University | ||||||
書誌情報 |
International Journal of Biological Sciences 巻 3, 号 6, p. 365-374, 発行日 2007 |
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年月次 | ||||||
2007 | ||||||
出版者名 | ||||||
出版者 | Ivyspring International Publisher | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 14492288 | |||||
関連サイト | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://www.biolsci.org/v03p0365.htm | http://www.biolsci.org/v03p0365.htm | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A novel method to link a nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was developed. The mRNA harboring amber stop codon at just downstream of initiation site was hybridized with hydrazide-modified ssDNA at upstream of coding region and was ligated to the DNA. This construct was then modified with 4-acetyl-phenylalanyl amber suppressor tRNA. This modified construct was fused with the nascent protein via the phenylalanine derivative when the mRNA uses the amber suppressor tRNA to decode the amber stop codon. The obtained fusion molecule was used successfully in selective enrichment experiments. It will be applicable for high-through-put screening in evolutionary protein engineering. In contrast to fusion molecules generated by other methods in which the protein is linked to genotype molecule through the C- terminus, our fusion molecule will serve to select a protein for which the C-terminus is essential to be active. | |||||
版 | ||||||
[著者版] | ||||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
アイテムID | ||||||
A1002279 |