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A bifunctional enzyme with L-fucokinase and GDP-L-fucose pyrophosphorylase activities salvages free L-fucose in Arabidopsis
https://sucra.repo.nii.ac.jp/records/13208
https://sucra.repo.nii.ac.jp/records/13208571ae3e7-ed5c-4f73-ae4b-e1b5a4567026
名前 / ファイル | ライセンス | アクション |
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A1002585.pdf (3.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-09 | |||||
タイトル | ||||||
タイトル | A bifunctional enzyme with L-fucokinase and GDP-L-fucose pyrophosphorylase activities salvages free L-fucose in Arabidopsis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
小竹, 敬久
× 小竹, 敬久× Hojo, Sachiko× Tajima, Noriaki× Matsuoka, Koji× Koyama, Tetsuo× 円谷, 陽一 |
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著者 ローマ字 | ||||||
Kotake, Toshihisa | ||||||
著者 ローマ字 | ||||||
Hojo, Sachiko | ||||||
著者 ローマ字 | ||||||
Tajima, Noriaki | ||||||
著者 ローマ字 | ||||||
Matsuoka, Koji | ||||||
著者 ローマ字 | ||||||
Koyama, Tetsuo | ||||||
著者 ローマ字 | ||||||
Tsumuraya, Yoichi | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属(別言語) | ||||||
Division of Life Science, Graduate School of Science and Engineering, Graduate School of Science and Engineering, Saitama University | ||||||
著者 所属(別言語) | ||||||
Division of Life Science, Graduate School of Science and Engineering, Graduate School of Science and Engineering, Saitama University | ||||||
著者 所属(別言語) | ||||||
Department of Biochemistry and Molecular Biology, Faculty of Science, Graduate School of Science and Engineering, Saitama University | ||||||
著者 所属(別言語) | ||||||
Division of Material Science, Graduate School of Science and Engineering, Saitama University | ||||||
書誌情報 |
JOURNAL OF BIOLOGICAL CHEMISTRY 巻 283, 号 13, p. 8125-8135, 発行日 2008 |
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年月次 | ||||||
2008-3 | ||||||
出版者名 | ||||||
出版者 | American Society for Biochemistry and Molecular Biology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00219258 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1074/jbc.M710078200 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Monomeric sugars generated during the metabolism of polysaccharides, glycoproteins, and glycolipids are imported to the cytoplasm and converted to respective nucleotide sugars via monosaccharide 1-phosphates, to be reutilized as activated sugars. Because L-fucose (L-Fuc) is activated mainly in the form of GDP derivatives in seed plants, the salvage reactions for L-Fuc are expected to be independent from those for Glc, Gal, L-arabinose, and glucuronic acid, which are activated as UDP-sugars. For this study we have identified, in the genomic data base of Arabidopsis, the gene (designated AtFKGP) of a bifunctional enzyme with similarity to both L-fucokinase and GDP-L-Fuc pyrophosphorylase. Recombinant AtFKGP (rAt-FKGP) expressed in Escherichia coli showed both L-fucokinase and GDP-L-Fuc pyrophosphorylase activities, generating GDP-L-Fuc from L-Fuc, ATP, and GTPas the starting substrates. Point mutations in rAtFKGPs at either Gly(133) or Gly(830) caused loss of GDP-L-Fuc pyrophosphorylase and L-fucokinase activity, respectively. The apparent Km values of L-fucokinase activity of rAtFKGP for L-Fuc and ATP were 1.0 and 0.45mM, respectively, and those of GDP-L-Fuc pyrophosphorylase activity for L-Fuc 1-phosphate and GTP were 0.052 and 0.17mM, respectively. The expression of AtFKGP was detected in most cell types of Arabidopsis, indicating that salvage reactions for free L-Fuc catalyzed by AtFKGP occur ubiquitously in Arabidopsis. Loss-of-function mutants with tDNA insertion in AtFKGP exhibited higher accumulation of free L-Fuc in the soluble fraction than the wild-type plant. These results indicate that AtFKGP is a bifunctional enzyme with L-fucokinase and GDP-L-Fuc pyrophosphorylase activities, which salvages free L-Fuc in Arabidopsis. | |||||
版 | ||||||
[著者版] | ||||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2008-04-09 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1002585 |