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Properties of family 79 beta-glucuronidases that hydrolyze beta-glucuronosyl and 4-O-methyl-beta-glucuronosyl residues of arabinogalactan-protein
https://sucra.repo.nii.ac.jp/records/13294
https://sucra.repo.nii.ac.jp/records/13294871e79f0-1582-47cf-ab00-fcbf49930bee
名前 / ファイル | ライセンス | アクション |
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A1002717.pdf (4.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-06-23 | |||||
タイトル | ||||||
タイトル | Properties of family 79 beta-glucuronidases that hydrolyze beta-glucuronosyl and 4-O-methyl-beta-glucuronosyl residues of arabinogalactan-protein | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | arabinogalactan-protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aspergillus niger | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | family 79 glycoside hydrolase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | beta-glucuronidase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Neurospora crassa | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transglycosylation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
古西, 智之
× 古西, 智之× 小竹, 敬久× Soraya, Dina× Matsuoka, Koji× Koyama, Tetsuo× Kaneko, Satoshi× Igarashi, Kiyohiko× Samejima, Masahiro× 円谷, 陽一 |
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著者 ローマ字 | ||||||
Konishi, Tomoyuki | ||||||
著者 ローマ字 | ||||||
Kotake, Toshihisa | ||||||
著者 ローマ字 | ||||||
Soraya, Dina | ||||||
著者 ローマ字 | ||||||
Matsuoka, Koji | ||||||
著者 ローマ字 | ||||||
Koyama, Tetsuo | ||||||
著者 ローマ字 | ||||||
Kaneko, Satoshi | ||||||
著者 ローマ字 | ||||||
Igarashi, Kiyohiko | ||||||
著者 ローマ字 | ||||||
Samejima, Masahiro | ||||||
著者 ローマ字 | ||||||
Tsumuraya, Yoichi | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属(別言語) | ||||||
Division of Life Science, Graduate School of Science and Engineering, Saitama University | ||||||
著者 所属(別言語) | ||||||
Division of Life Science, Graduate School of Science and Engineering, Saitama University | ||||||
著者 所属(別言語) | ||||||
Division of Life Science, Graduate School of Science and Engineering, Saitama University | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
Division of Life Science, Graduate School of Science and Engineering, Saitama University | ||||||
書誌情報 |
CARBOHYDRATE RESEARCH 巻 343, 号 7, p. 1191-1201, 発行日 2008 |
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年月次 | ||||||
2008-5 | ||||||
出版者名 | ||||||
出版者 | Elsevier Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00086215 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.carres.2008.03.004 | |||||
関連サイト | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://www.sciencedirect.com/science/journal/00086215 | http://www.sciencedirect.com/science/journal/00086215 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The carbohydrate moieties of arabinogalactan-proteins (AGPs), which are mainly composed of Gal, L-Ara, GlcA, and 4Me-GlcA residues, are essential for the physiological functions of these proteoglycans in higher plants. For this study, we have identified two genes encoding family 79 beta-glueuronidases, designated AnGlcAase and NcGlcAase, in Aspergillus niger and Neurospora crassa, respectively, based on the amino acid sequence of a native beta-glucuronidase purified from a commercial pectolytic enzyme preparation from A. niger. Although the deduced protein sequences of AnGlcAase and NcGlcAase were highly similar, the recombinant enzymes expressed in Pichia pastoris exhibited distinct substrate specificity toward 4-Me-GlcA residues of AGPs: recombinant ADGlcAase (rAnGlcAase) substantially liberated both GlcA and 4-Me-GlcA residues from radish AGPs, whereas recombinant NcGlcAase (rNcGlcAase) activity on the 4-Me-GlcA residues of AGPs was very low. Maximum activity of rAnGlcAase hydrolyzing PNP beta-GlcA occurred at pH 3.0-4.0, whereas the maximum rNcGlcAase activity was at pH 6.0. The apparent K. values of rAnGlcAase were 30.4 mu M for PNP beta-GlcA and 422 mu M for beta-GIcA-(1 -> 6)-Gal, and those of rNcGlcAase were 38.3 mu M and 378 mu M, respectively. Similar to the native enzyme, rAnGlcAase was able to catalyze the transglycosylation of GlcA residues from PNP beta-GlcA to various monosaccharide acceptors such as Glc, Gal, and Xyl. We propose that both AnGlcAase and NcGlcAase are instances of a novel type of beta-glucuronidase with the capacity to hydrolyze beta-GlcA and 4-Me-beta-GlcA residues of AGPs, although they differ significantly in their preferences. (c) 2008 Elsevier Ltd. All rights reserved. | |||||
版 | ||||||
[著者版] | ||||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2008-06-23 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1002717 |