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Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry
https://sucra.repo.nii.ac.jp/records/13336
https://sucra.repo.nii.ac.jp/records/13336b5eec8d6-2970-4b1b-a167-deaefea5234e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
A1002780.pdf (586.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2008-09-03 | |||||
| タイトル | ||||||
| タイトル | Difference in metabolite levels between photoautotrophic and photomixotrophic cultures of Synechocystis sp PCC 6803 examined by capillary electrophoresis electrospray ionization mass spectrometry | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CE/MS | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cyanobacteria | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | glucose | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | metabolome | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | photomixotrophy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | pmgA | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Takahashi, Hideyuki
× Takahashi, Hideyuki× Uchimiya, Hirofumi× 日原, 由香子 |
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| 著者 ローマ字 | ||||||
| 値 | Takahashi, Hideyuki | |||||
| 著者 ローマ字 | ||||||
| 値 | Uchimiya, Hirofumi | |||||
| 著者 ローマ字 | ||||||
| 値 | Hihara, Yukako | |||||
| 著者 所属 | ||||||
| 著者 所属 | ||||||
| 著者 所属 | ||||||
| 値 | 埼玉大学理学部分子生物学科 | |||||
| 著者 所属(別言語) | ||||||
| 著者 所属(別言語) | ||||||
| 著者 所属(別言語) | ||||||
| 値 | Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University | |||||
| 書誌情報 |
JOURNAL OF EXPERIMENTAL BOTANY 巻 59, 号 11, p. 3009-3018, 発行日 2008 |
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| 年月次 | ||||||
| 値 | 2008-8 | |||||
| 出版者名 | ||||||
| 出版者 | Oxford University Press | |||||
| 収録物識別子 | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00220957 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1093/jxb/ern157 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Capillary electrophoresis mass spectrometry (CE/MS) was applied for the comprehensive survey of changes in the amounts of metabolites upon the shift from photoautotrophic to photomixotrophic conditions in Synechocystis sp. PCC 6803. When glucose was added to the photoautotrophically grown culture, the increase in the metabolites for the oxidative pentose phosphate (OPP) pathway and glycolysis, together with the decrease in those for the Calvin cycle, was observed. Concomitantly, the increase in respiratory activity and the decrease in photosynthetic activity took place in the wild-type cells. In the pmgA-disrupted mutant that shows growth inhibition under photomixotrophic conditions, lower enzymatic activities of the OPP pathway and higher photosynthetic activity were observed, irrespective of trophic conditions. These defects brought about metabolic disorders such as a decrease in ATP and NADPH contents, a failure in the activation of respiratory activity, and the aberrant accumulation of isocitrate under photomixotrophic but not under photoautotrophic conditions. A delicate balancing of the carbon flow between the Calvin cycle and the OPP pathway seems indispensable for growth specifically under photomixotrophic conditions and PmgA is likely to be involved in the regulation. | |||||
| 版 | ||||||
| 値 | [著者版] | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 作成日 | ||||||
| 日付 | 2008-09-03 | |||||
| 日付タイプ | Created | |||||
| アイテムID | ||||||
| 値 | A1002780 | |||||
