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The Asymmetric Trimeric Architecture of [2Fe-2S] IscU: Implications for Its Scaffolding during Iron-Sulfur Cluster Biosynthesis
https://sucra.repo.nii.ac.jp/records/13354
https://sucra.repo.nii.ac.jp/records/13354078c89b1-14e0-4c3f-8eda-6e9fe06449a4
名前 / ファイル | ライセンス | アクション |
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A1002800.pdf (3.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-12-03 | |||||
タイトル | ||||||
タイトル | The Asymmetric Trimeric Architecture of [2Fe-2S] IscU: Implications for Its Scaffolding during Iron-Sulfur Cluster Biosynthesis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fe-S cluster | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biosynthesis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ISC machinery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | scaffold | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | X-ray crystallography | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Shimomura, Yoshimitsu
× Shimomura, Yoshimitsu× Wada, Kei× Fukuyama, Keiichi× 高橋, 康弘 |
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著者 ローマ字 | ||||||
Shimomura, Yoshimitsu | ||||||
著者 ローマ字 | ||||||
Wada, Kei | ||||||
著者 ローマ字 | ||||||
Fukuyama, Keiichi | ||||||
著者 ローマ字 | ||||||
Takahashi, Yasuhiro | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属(別言語) | ||||||
Department of Biological Sciences, Graduate School of Science, Osaka University | ||||||
著者 所属(別言語) | ||||||
Department of Biological Sciences, Graduate School of Science, Osaka University | ||||||
著者 所属(別言語) | ||||||
Department of Biological Sciences, Graduate School of Science, Osaka University | ||||||
著者 所属(別言語) | ||||||
Division of Life Science, Graduate School of Science and Engineering, Saitama University | ||||||
書誌情報 |
JOURNAL OF MOLECULAR BIOLOGY 巻 383, 号 1, p. 133-143, 発行日 2008 |
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年月次 | ||||||
2008-10 | ||||||
出版者名 | ||||||
出版者 | ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00222836 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.jmb.2008.08.015 | |||||
関連サイト | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://www.sciencedirect.com/science/journal/00222836 | http://www.sciencedirect.com/science/journal/00222836 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | IscU is a key component of the ISC machinery and is involved in the biogenesis of iron-sulfur (Fe-S) proteins. IscU serves as a scaffold for assembly of a nascent Fe-S cluster prior to its delivery to an apo protein. Here, we report the first crystal structure of IscU with a bound [2Fe-2S] cluster from the hyperthermophilic bacterium Aquifex aeolicus, determined at a resolution of 2.3 angstrom, using multiwavelength anomalous diffraction of the cluster. The holo IscU formed a novel asymmetric trimer that harbored only one [2Fe-2S] cluster. One iron atom of the cluster was coordinated by the S-gamma atom of Cys36 and the N-epsilon atom of Hs106, and the other was coordinated by the S-gamma atoms of Cys63 and Cys107 on the surface of just one of the protomers. However, the cluster was buried inside the trimer between the neighboring protomers. The three protomers were conformationally distinct from one another and associated around a noncrystallographic pseudo-3-fold axis. The three flexible loop regions carrying the ligand-binding residues (Cys36, Cys63,His106 and Cys107) and the N-terminal alpha 1 helices were positioned at the interfaces and underwent substantial conformational rearrangement, which stabilized the association of the asymmetric trimer. This unique trimeric A. aeolicus holo-IscU architecture was clearly distinct from other known monomeric apo-IscU/SufU structures, indicating that asymmetric trimer organization, as well as its association/dissociation, would be involved in the scaffolding function of IscU. (C) 2008 Elsevier Ltd. All rights reserved. | |||||
版 | ||||||
[著者版] | ||||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2008-12-03 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1002800 |