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Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis
https://sucra.repo.nii.ac.jp/records/13407
https://sucra.repo.nii.ac.jp/records/134072593316c-35af-43ca-9d2c-dae60cdbc224
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
A1002857.pdf (488.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-05-25 | |||||
| タイトル | ||||||
| タイトル | Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | glycerolipid synthesis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | sn-glycerol-3-phosphate acyltransferase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PlsX | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PlsY | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | acyl-phosphate | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | lysophosphatidic acid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | LPA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phosphatidic acid | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Bacillus subtilis | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Hara, Yoshinori
× Hara, Yoshinori× Seki, Masahide× 松岡, 聡× 原, 弘志× Yamashita, Atsushi× 松本, 幸次 |
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| 著者 ローマ字 | ||||||
| 値 | Hara, Yoshinori | |||||
| 著者 ローマ字 | ||||||
| 値 | Seki, Masahide | |||||
| 著者 ローマ字 | ||||||
| 値 | Matsuoka, Satoshi | |||||
| 著者 ローマ字 | ||||||
| 値 | Hara, Hiroshi | |||||
| 著者 ローマ字 | ||||||
| 値 | Yamashita, Atsushi | |||||
| 著者 ローマ字 | ||||||
| 値 | Matsumoto, Kouji | |||||
| 著者 所属 | ||||||
| 著者 所属 | ||||||
| 著者 所属 | ||||||
| 値 | 埼玉大学大学院理工学研究科生命科学部門 | |||||
| 著者 所属 | ||||||
| 値 | 埼玉大学大学院理工学研究科生命科学部門 | |||||
| 著者 所属 | ||||||
| 著者 所属 | ||||||
| 値 | 埼玉大学大学院理工学研究科生命科学部門 | |||||
| 著者 所属(別言語) | ||||||
| 著者 所属(別言語) | ||||||
| 著者 所属(別言語) | ||||||
| 値 | Graduate School of Science and Engineering, Saitama University | |||||
| 著者 所属(別言語) | ||||||
| 値 | Graduate School of Science and Engineering, Saitama University | |||||
| 著者 所属(別言語) | ||||||
| 著者 所属(別言語) | ||||||
| 値 | Graduate School of Science and Engineering, Saitama University | |||||
| 書誌情報 |
GENES & GENETIC SYSTEMS 巻 83, 号 6, p. 433-442, 発行日 2008 |
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| 年月次 | ||||||
| 値 | 2008-12 | |||||
| 出版者名 | ||||||
| 出版者 | the Genetics Society of Japan | |||||
| 収録物識別子 | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13417568 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | info:doi/10.1266/ggs.83.433 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The gene responsible for the first acylation of sn-glycerol-3-phosphate (G3P) in Bacillus subtilis has not yet been determined with certainty. The product of this first acylation, lysophosphatidic acid (LPA), is subsequently acylated again to form phosphatidic acid (PA), the primary precursor to membrane glycerolipids. A novel G3P acyltransferase (GPAT), the gene product of plsY, which uses acylphosphate formed by the plsX gene product, has recently been found to synthesize LPA in Streptococcus pneumoniae. We found that in B. subtilis growth arrests after repression of either a plsY homologue or a plsX homologue were overcome by expression of E. coli plsB, which encodes an acyl-acylcarrier protein (acyl-ACP)dependent GPAT, although in the case of plsX repression a high level of plsB expression was required. B. subtilis has, therefore, a capability to use the acyl-ACP dependent GPAT of PIsB. Simultaneous expression of plsY and plsX suppressed the glycerol requirement of a strict glycerol auxotrophic derivative of the E. coli plsB26 mutant, although either one alone did not. Membrane fractions from B. subtilis cells catalyzed palmitoylphosphate-dependent acylation of [C-14]- labeled G3P to synthesize [C-14] -labeled LPA, whereas those from Delta plsY cells did not. The results indicate unequivocally that PIsY is an acyl-phosphate dependent GPAT. Expression of plsX corrected the glycerol auxotrophy of a Delta ygiH (the deleted allele of an E. coli homologue of plsY) derivative of BB26-36 (plsB26 plsX50), suggesting an essential role of plsX other than substrate supply for acylphosphate dependent LPA synthesis. Two-hybrid examinations suggested that PIsY is associated with PlsX and that each may exist in multimeric form. | |||||
| 版 | ||||||
| 値 | [出版社版] | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 資源タイプ | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | text | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 作成日 | ||||||
| 日付 | 2009-05-25 | |||||
| 日付タイプ | Created | |||||
| アイテムID | ||||||
| 値 | A1002857 | |||||
