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Visualization of BrdU-labelled DNA in cyanobacterial cells by Hilbert differential contrast transmission electron microscopy
https://sucra.repo.nii.ac.jp/records/13423
https://sucra.repo.nii.ac.jp/records/13423da5aff06-4d24-4c22-b81d-3717707abea0
名前 / ファイル | ライセンス | アクション |
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A1002881.pdf (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-06-05 | |||||
タイトル | ||||||
タイトル | Visualization of BrdU-labelled DNA in cyanobacterial cells by Hilbert differential contrast transmission electron microscopy | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | BrdU labelling | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cyanobacteria | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | native DNA structure | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | phase-contrast electron microscopy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rapid freezing | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Nitta, K.
× Nitta, K.× Nagayama, K.× Danev, R.× 金子, 康子 |
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著者 ローマ字 | ||||||
Nitta, K. | ||||||
著者 ローマ字 | ||||||
Nagayama, K. | ||||||
著者 ローマ字 | ||||||
Danev, R. | ||||||
著者 ローマ字 | ||||||
Kaneko, Yasuko | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
Graduate School of Science and Engineering, Saitama University | ||||||
書誌情報 |
JOURNAL OF MICROSCOPY-OXFORD 巻 234, 号 2, p. 118-123, 発行日 2009 |
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年月次 | ||||||
2009-5 | ||||||
出版者名 | ||||||
出版者 | WILEY-BLACKWELL PUBLISHING | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00222720 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1111/j.1365-2818.2009.03162.x | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have attempted to observe the native shape of DNA in rapidly frozen whole cyanobacterial cells through 5-bromo-2-deoxyuridine (BrdU) incorporation and visualization with a Hilbert differential contrast transmission electron microscopy (HDC TEM). The incorporation of BrdU into the DNA of Synechococcus elongatus PCC 7942 was confirmed with fluorescently labelled anti-BrdU antibodies and through EDX analysis of ultra-thin sections. HDC TEM observed cells that had incorporated BrdU into their DNA exhibited electron dense areas at the location corresponding to fluorescently labelled BrdU. Since various strings and strands were observed in high contrast with the HDC TEM, we conclude that the method promises to allow us to identify and understand bulk structural changes of the in vivo DNA and the nucleoid through observation at high resolution. | |||||
版 | ||||||
[著者版] | ||||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2009-06-05 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1002881 |