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Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum
https://sucra.repo.nii.ac.jp/records/14342
https://sucra.repo.nii.ac.jp/records/14342a64176a8-a7da-4028-a748-e3135b518523
名前 / ファイル | ライセンス | アクション |
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A1002070.pdf (835.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-03-23 | |||||
タイトル | ||||||
タイトル | Transcript profiling of salinity stress responses by large-scale expressed sequence tag analysis in Mesembryanthemum crystallinum | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | crassulacean acid metabolism | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | salinity stress | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | common ice plant | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
是枝, 晋
× 是枝, 晋 |
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著者 ローマ字 | ||||||
Kore-eda, Shin | ||||||
著者 ローマ字 | ||||||
Cushman, Mary Ann | ||||||
著者 ローマ字 | ||||||
Akselrod, Inna | ||||||
著者 ローマ字 | ||||||
Bufford, Davina | ||||||
著者 ローマ字 | ||||||
Fredrickson, Monica | ||||||
著者 ローマ字 | ||||||
Clark, Elizabeth | ||||||
著者 ローマ字 | ||||||
Cushman, John. C | ||||||
著者 所属 | ||||||
埼玉大学科学分析支援センター | ||||||
著者 所属(別言語) | ||||||
Molecular Analysis and Life Science Center, Saitama University | ||||||
書誌情報 |
Gene 巻 341, 号 27, p. 83-92, 発行日 2004 |
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年月次 | ||||||
2004-10 | ||||||
出版者名 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03781119 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.gene.2004.06.037 | |||||
関連サイト | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://www.sciencedirect.com/science/journal/03781119 | http://www.sciencedirect.com/science/journal/03781119 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The common ice plant, Mesembryanthemum crystallinum, is a halophytic (salt-loving) member of the Aizoaceae, which switches from C3 photosynthesis to Crassulacean acid metabolism (CAM) when exposed to salinity or water-deficit stress. CAM is a metabolic adaptation of photosynthetic carbon fixation that improves water use efficiency by shifting net CO2 uptake to the night, thereby reducing transpirational water loss. To improve our understanding of the molecular genetic underpinnings and control mechanisms for Crassulacean acid metabolism (CAM) and other salinity stress response adaptations, a total of 9,733 expressed sequence tags (ESTs) from cDNAs derived from leaf tissues of well-watered and salinity-stressed (0.5 M NaCl for 30 h and 48 h) were characterized. Clustering and assembly of these ESTs resulted in the identification of a total of 3,676 tentative unique gene sequences (1,249 tentative consensus sequences and 2,427 singleton ESTs) expressed in leaves of ice plant under unstressed and salinity stressed conditions. The same number (2,782) of ESTs from each library (total = 8,346 ESTs) were randomly selected and analyzed to compare expression profiles among the control and salt stressed leaf tissues. EST frequencies for transcripts encoding CAM-related enzymes, pathogenesis-related, senescence-associated, cell death-related, and stress-related proteins such as heat shock proteins, chaperones, early light-inducible proteins, ion homeostasis, antioxidative stress, detoxification, and biosynthetic enzymes for osmoprotectants increased 2-12-fold in cDNA libraries constructed from salt stressed plants. In contrast, the frequency of ESTs encoding light-harvesting and photosystem complexes and C3 photosynthetic enzymes decreased four-fold overall following salinity stress with transcripts for Ribulose bisphosphate carboxylase/oxygenase subunits decreasing seven-fold. Moreover, stressed plants contained a higher percentage of ESTs encoding novel and/or functionally unknown proteins. The rapid discovery of both known and unknown genes related to stress responses in M. crystallinum demonstrates the great utility of EST analysis in unraveling the complex set of adaptive mechanisms contributing to water use efficiency (CAM) and salinity tolerance. | |||||
版 | ||||||
[著者版] | ||||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2007-03-05 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1002070 |