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Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals
https://sucra.repo.nii.ac.jp/records/14475
https://sucra.repo.nii.ac.jp/records/14475402b14ef-aa11-4bfd-97e0-567cdd8f195b
名前 / ファイル | ライセンス | アクション |
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A3000387.pdf (2.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-03-09 | |||||
タイトル | ||||||
タイトル | Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
大倉, 正道
× 大倉, 正道× SASAKI, Takuya× SADAKARI, Junko× 安藤, 恵子× KAGAWA-NAGAMURA, Yuko× KOBAYASHI, Chiaki× IKEGAYA, Yuji× 中井, 淳一 |
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著者 ローマ字 | ||||||
OHKURA, Masamichi | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
GENGYO-ANDO, Keiko | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
著者 ローマ字 | ||||||
NAKAI, Junichi | ||||||
著者 所属 | ||||||
Brain Science Institute, Saitama University | ||||||
著者 所属 | ||||||
Brain Science Institute, Saitama University | ||||||
著者 所属 | ||||||
Brain Science Institute, Saitama University | ||||||
著者 所属 | ||||||
Brain Science Institute, Saitama University | ||||||
著者 所属 | ||||||
Brain Science Institute, Saitama University | ||||||
著者 所属 | ||||||
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, University of Tokyo | ||||||
著者 所属 | ||||||
Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, University of Tokyo | ||||||
著者 所属 | ||||||
Brain Science Institute, Saitama University | ||||||
書誌情報 |
PLoS ONE 巻 7, 号 12, p. 1-10, 発行日 2012 |
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年月次 | ||||||
2012-12 | ||||||
出版者名 | ||||||
出版者 | Public Library of Science (PLoS) | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1371/journal.pone.0051286 | |||||
識別番号 その他 | ||||||
内容記述タイプ | Other | |||||
内容記述 | e51286 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Imaging the activities of individual neurons with genetically encoded Ca2+ indicators (GECIs) is a promising method for understanding neuronal network functions. Here, we report GECIs with improved neuronal Ca2+ signal detectability, termed G-CaMP6 and G-CaMP8. Compared to a series of existing G-CaMPs, G-CaMP6 showed fairly high sensitivity and rapid kinetics, both of which are suitable properties for detecting subtle and fast neuronal activities. G-CaMP8 showed a greater signal (Fmax/Fmin = 38) than G-CaMP6 and demonstrated kinetics similar to those of G-CaMP6. Both GECIs could detect individual spikes from pyramidal neurons of cultured hippocampal slices or acute cortical slices with 100% detection rates, demonstrating their superior performance to existing GECIs. Because G-CaMP6 showed a higher sensitivity and brighter baseline fluorescence than G-CaMP8 in a cellular environment, we applied G-CaMP6 for Ca2+ imaging of dendritic spines, the putative postsynaptic sites. By expressing a G-CaMP6-actin fusion protein for the spines in hippocampal CA3 pyramidal neurons and electrically stimulating the granule cells of the dentate gyrus, which innervate CA3 pyramidal neurons, we found that sub-threshold stimulation triggered small Ca2+ responses in a limited number of spines with a low response rate in active spines, whereas supra-threshold stimulation triggered large fluorescence responses in virtually all of the spines with a 100% activity rate. | |||||
版 | ||||||
[出版社版] | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2015-03-06 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A3000387 |