WEKO3
アイテム
{"_buckets": {"deposit": "a690f657-ffb0-46fb-a28d-3d5345164fe8"}, "_deposit": {"created_by": 3, "id": "16640", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "16640"}, "status": "published"}, "_oai": {"id": "oai:sucra.repo.nii.ac.jp:00016640", "sets": ["634"]}, "author_link": ["27125", "27124", "27122", "27126", "27123", "17549"], "item_120_alternative_title_1": {"attribute_name": "タイトル(別言語)", "attribute_value_mlt": [{"subitem_alternative_title": "Construction and Application of Peptide-Library Generated by YLBS"}]}, "item_120_biblio_info_8": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2002", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "134", "bibliographicPageStart": "133", "bibliographicVolumeNumber": "2", "bibliographic_titles": [{"bibliographic_title": "埼玉大学地域共同研究センター紀要"}, {"bibliographic_title": "Report of Cooperative Research Center, Saitama University", "bibliographic_titleLang": "en"}]}]}, "item_120_date_31": {"attribute_name": "作成日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2009-06-24", "subitem_date_issued_type": "Created"}]}, "item_120_description_19": {"attribute_name": "概要", "attribute_value_mlt": [{"subitem_description": "Peptides have a big potential to be used for biologically active molecules such as hormone, antibiotics, inhibitors and others. Therefore, they are one of the most important substances targeted by Evolutionary Molecular Engineering. The possibility of building peptide-coding DNA libraries, beginning from 20 species of amino acid-monomer blocks, by use of YLBS technology was examined. Three/ four steps of Y-ligation were performed, providing y3 N 4 DNA libraries corresponding to peptides of 8-mers/16-mers, respectively. Y3 was confirmed to be really successful in containing the whole set of 8mers. Tenacious problems of deletion and uneven appearance of blocks were challenged and fruitfully solved by introducing an operation which does not depend on the second-strand cutting of DNA by restriction enzymes or by starting with preferential blocks for T4 RNA ligation.", "subitem_description_type": "Other"}]}, "item_120_description_29": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"subitem_description": "text", "subitem_description_type": "Other"}]}, "item_120_description_30": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_120_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.24561/00016634", "subitem_identifier_reg_type": "JaLC"}]}, "item_120_other_language_22": {"attribute_name": "その他の言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_120_publisher_11": {"attribute_name": "出版者名", "attribute_value_mlt": [{"subitem_publisher": "埼玉大学地域共同研究センター"}]}, "item_120_relation_7": {"attribute_name": "著者 外部リンク", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "\u0026EMPTY\u0026"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "\u0026EMPTY\u0026", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "\u0026EMPTY\u0026"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "\u0026EMPTY\u0026", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "\u0026EMPTY\u0026"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "\u0026EMPTY\u0026", "subitem_relation_type_select": "URI"}}, {"subitem_relation_name": [{"subitem_relation_name_text": "http://s-read.saitama-u.ac.jp/researchers/pages/researcher/aZUKsNBx"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://s-read.saitama-u.ac.jp/researchers/pages/researcher/aZUKsNBx", "subitem_relation_type_select": "URI"}}]}, "item_120_source_id_14": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "13474758", "subitem_source_identifier_type": "ISSN"}]}, "item_120_text_3": {"attribute_name": "著者 ローマ字", "attribute_value_mlt": [{"subitem_text_value": "Kobayashi, Teruaki"}, {"subitem_text_value": "Narasaki, Shinsuke"}, {"subitem_text_value": "Kinoshita, Yasunori"}, {"subitem_text_value": "Nemoto, Naoto"}, {"subitem_text_value": "Fukushima, Hideo"}, {"subitem_text_value": "Nishigaki, Koichi"}]}, "item_120_text_32": {"attribute_name": "アイテムID", "attribute_value_mlt": [{"subitem_text_value": "KY-AA11808968-02-34"}]}, "item_120_text_35": {"attribute_name": "公開日(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "Jun 24, 2009 09:00:00"}]}, "item_120_text_36": {"attribute_name": "記録日(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "2009-06-24"}]}, "item_120_text_37": {"attribute_name": "最終更新日(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "Apr 4, 2014 11:09:52"}]}, "item_120_text_38": {"attribute_name": "公開日(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "Jun 24, 2009 09:00:00"}]}, "item_120_text_39": {"attribute_name": "更新履歴(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "Apr 4, 2014 著者 を変更"}, {"subitem_text_value": "Sep 4, 2009 著者 を変更"}, {"subitem_text_value": "Jun 25, 2009 抄録 を変更"}, {"subitem_text_value": "Jun 25, 2009 インデックス を変更"}, {"subitem_text_value": "Jun 24, 2009 フリーキーワード, インデックス, 抄録 を変更"}]}, "item_120_text_4": {"attribute_name": "著者 所属", "attribute_value_mlt": [{"subitem_text_value": "株式会社ジェンコム研究開発部"}, {"subitem_text_value": "埼玉大学工学部機能材料工学科"}, {"subitem_text_value": "埼玉大学工学部機能材料工学科"}, {"subitem_text_value": "株式会社ジェンコム研究開発部"}, {"subitem_text_value": "株式会社ジェンコム研究開発部"}, {"subitem_text_value": "埼玉大学工学部機能材料工学科"}]}, "item_120_text_40": {"attribute_name": "登録者(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "sucra_jim4"}]}, "item_120_text_41": {"attribute_name": "閲覧数(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "1037"}]}, "item_120_text_42": {"attribute_name": "ダウンロード数(XooNIps)", "attribute_value_mlt": [{"subitem_text_value": "622"}]}, "item_120_text_43": {"attribute_name": "XooNIps_インデックス", "attribute_value_mlt": [{"subitem_text_value": "sucra_jim4|Public/埼玉大学/理工学研究科|Public/埼玉大学/理工学研究科/物質科学部門|Public/ジャンル別/研究紀要/埼玉大学/地域共同研究センター紀要|Public/主題別/複合新領域/ゲノム科学|Public/主題別/生物学/生物科学/進化生物学"}]}, "item_120_text_44": {"attribute_name": "XooNIps_ITEM_KEY", "attribute_value_mlt": [{"subitem_text_value": "4243"}]}, "item_120_text_5": {"attribute_name": "著者 所属(別言語)", "attribute_value_mlt": [{"subitem_text_value": "Department of Research and Development, GenCom Co., Ltd."}, {"subitem_text_value": "Department of Functional Materials Science, Saitama University"}, {"subitem_text_value": "Department of Functional Materials Science, Saitama University"}, {"subitem_text_value": "Department of Research and Development, GenCom Co., Ltd."}, {"subitem_text_value": "Department of Research and Development, GenCom Co., Ltd."}, {"subitem_text_value": "Department of Functional Materials Science, Saitama University"}]}, "item_120_text_9": {"attribute_name": "年月次", "attribute_value_mlt": [{"subitem_text_value": "2001"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "小林, 輝章"}], "nameIdentifiers": [{"nameIdentifier": "27122", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "楢崎, 真介"}], "nameIdentifiers": [{"nameIdentifier": "27123", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "木下, 保則"}], "nameIdentifiers": [{"nameIdentifier": "27124", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "根本, 直人"}], "nameIdentifiers": [{"nameIdentifier": "17549", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "aZUKsNBx", "nameIdentifierScheme": "研究者総覧", "nameIdentifierURI": "http://s-read.saitama-u.ac.jp/researchers/pages/researcher/aZUKsNBx"}]}, {"creatorNames": [{"creatorName": "福島, 秀郎"}], "nameIdentifiers": [{"nameIdentifier": "27125", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "西垣, 功一"}, {"creatorName": "ニシガキ, コウイチ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "27126", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KY-AA11808968-02-34.pdf", "filesize": [{"value": "103.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 103300.0, "url": {"label": "KY-AA11808968-02-34.pdf", "url": "https://sucra.repo.nii.ac.jp/record/16640/files/KY-AA11808968-02-34.pdf"}, "version_id": "9f7bb576-b6aa-4633-85c2-3352dd216dce"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Y-ligation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "block shuffling", "subitem_subject_scheme": "Other"}, {"subitem_subject": "evolutionary protein engineering", "subitem_subject_scheme": "Other"}, {"subitem_subject": "peptide", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "YLBSによるペプチドライブラリーの作成と利用", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "YLBSによるペプチドライブラリーの作成と利用"}]}, "item_type_id": "120", "owner": "3", "path": ["634"], "permalink_uri": "https://doi.org/10.24561/00016634", "pubdate": {"attribute_name": "公開日", "attribute_value": "2009-06-24"}, "publish_date": "2009-06-24", "publish_status": "0", "recid": "16640", "relation": {}, "relation_version_is_last": true, "title": ["YLBSによるペプチドライブラリーの作成と利用"], "weko_shared_id": -1}
YLBSによるペプチドライブラリーの作成と利用
https://doi.org/10.24561/00016634
https://doi.org/10.24561/00016634a19e9bd4-fde7-406a-bb3b-76b6c1811930
名前 / ファイル | ライセンス | アクション |
---|---|---|
KY-AA11808968-02-34.pdf (103.3 kB)
|
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2009-06-24 | |||||
タイトル | ||||||
タイトル | YLBSによるペプチドライブラリーの作成と利用 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Y-ligation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | block shuffling | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | evolutionary protein engineering | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | peptide | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.24561/00016634 | |||||
ID登録タイプ | JaLC | |||||
タイトル(別言語) | ||||||
その他のタイトル | Construction and Application of Peptide-Library Generated by YLBS | |||||
著者 |
小林, 輝章
× 小林, 輝章× 楢崎, 真介× 木下, 保則× 根本, 直人× 福島, 秀郎× 西垣, 功一 |
|||||
著者 ローマ字 | ||||||
Kobayashi, Teruaki | ||||||
著者 ローマ字 | ||||||
Narasaki, Shinsuke | ||||||
著者 ローマ字 | ||||||
Kinoshita, Yasunori | ||||||
著者 ローマ字 | ||||||
Nemoto, Naoto | ||||||
著者 ローマ字 | ||||||
Fukushima, Hideo | ||||||
著者 ローマ字 | ||||||
Nishigaki, Koichi | ||||||
著者 所属 | ||||||
株式会社ジェンコム研究開発部 | ||||||
著者 所属 | ||||||
埼玉大学工学部機能材料工学科 | ||||||
著者 所属 | ||||||
埼玉大学工学部機能材料工学科 | ||||||
著者 所属 | ||||||
株式会社ジェンコム研究開発部 | ||||||
著者 所属 | ||||||
株式会社ジェンコム研究開発部 | ||||||
著者 所属 | ||||||
埼玉大学工学部機能材料工学科 | ||||||
著者 所属(別言語) | ||||||
Department of Research and Development, GenCom Co., Ltd. | ||||||
著者 所属(別言語) | ||||||
Department of Functional Materials Science, Saitama University | ||||||
著者 所属(別言語) | ||||||
Department of Functional Materials Science, Saitama University | ||||||
著者 所属(別言語) | ||||||
Department of Research and Development, GenCom Co., Ltd. | ||||||
著者 所属(別言語) | ||||||
Department of Research and Development, GenCom Co., Ltd. | ||||||
著者 所属(別言語) | ||||||
Department of Functional Materials Science, Saitama University | ||||||
書誌情報 |
埼玉大学地域共同研究センター紀要 en : Report of Cooperative Research Center, Saitama University 巻 2, p. 133-134, 発行日 2002 |
|||||
年月次 | ||||||
2001 | ||||||
出版者名 | ||||||
出版者 | 埼玉大学地域共同研究センター | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13474758 | |||||
概要 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Peptides have a big potential to be used for biologically active molecules such as hormone, antibiotics, inhibitors and others. Therefore, they are one of the most important substances targeted by Evolutionary Molecular Engineering. The possibility of building peptide-coding DNA libraries, beginning from 20 species of amino acid-monomer blocks, by use of YLBS technology was examined. Three/ four steps of Y-ligation were performed, providing y3 N 4 DNA libraries corresponding to peptides of 8-mers/16-mers, respectively. Y3 was confirmed to be really successful in containing the whole set of 8mers. Tenacious problems of deletion and uneven appearance of blocks were challenged and fruitfully solved by introducing an operation which does not depend on the second-strand cutting of DNA by restriction enzymes or by starting with preferential blocks for T4 RNA ligation. | |||||
その他の言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2009-06-24 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
KY-AA11808968-02-34 |