@phdthesis{oai:sucra.repo.nii.ac.jp:00010313,
 author = {望月, 佑樹},
 month = {},
 note = {69 p., Chapter 1: Introduction 1
1-1. Peptide aptamer 1
1-2. Disulfide-rich peptides 4
1-3. In vitro display technologies 7
1-4. cDNA display 10
1-5. Aims of this study 14
Chapter 2: Improvement of in vitro selection/evolution system by cDNA display method 15
2-1. Increasing the library size in cDNA display by optimizing purification procedures 15
2-1-1. Introduction 15
2-1-2. Materials and methods 17
2-1-3. Results and discussion 18
2-2. A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin-linker. 24
2-2-1. Introduction 24
2-2-2. Materials and methods 25
2-2-3. Results and discussion 28
2-3. Conclusions 32
Chapter 3: In vitro selection of disulfide-rich peptides against amino groups by cDNA display method 33
3-1. Introduction 33
3-2. Materials and methods 35
3-2-1. Library construction 35
3-2-2. SBP-linker synthesis 37
3-2-3. cDNA display preparation from library DNAs 39
3-2-4. Affinity selection 41
3-2-5. Peptides 43
3-2-6. Trypsin digestion and mass spectrometry analysis 44
3-2-7. Binding assay against amino group modified magnetic beads 44
3-2-8. Binding assay against a glass slide 45
3-2-9. Binding assay against agarose beads 45
3-2-10. Circular dichroism analysis 46
3-3. Results and discussion 47
3-3-1. In vitro selection of amino group binding peptides 48
3-3-2. Binding assay by a pull-down method 49
3-3-3. Analysis of the relationship between disulfide connectivity and function 50
3-3-4. Binding assay against amino groups on several kinds of solid supports 54
3-3-5. Binding assay of CP1 (2SS)-α derivative peptides 55
3-3-6. Secondary structure analysis 57
3-4. Conclusions 59
Chapter 4: Overall conclusions 60
References 61
Acknowledgments 69, 主指導教員 : 根本直人, text, application/pdf},
 school = {埼玉大学},
 title = {架橋構造を有する分子認識ペプチドの試験管内淘汰に関する研究},
 year = {2014},
 yomi = {モチヅキ, ユウキ}
}