{"created":"2023-05-15T15:23:28.865809+00:00","id":10313,"links":{},"metadata":{"_buckets":{"deposit":"00f3ff35-b62d-4a2b-b210-cf29b236c4ad"},"_deposit":{"created_by":15,"id":"10313","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10313"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00010313","sets":["94:429:431:432:504"]},"author_link":[],"item_113_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"}}]},"item_113_date_35":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-02-04","subitem_date_issued_type":"Created"}]},"item_113_date_granted_20":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-24"}]},"item_113_degree_grantor_22":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"埼玉大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"12401","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_113_degree_name_21":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(工学)"}]},"item_113_description_13":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_description":"69 p.","subitem_description_type":"Other"}]},"item_113_description_24":{"attribute_name":"目次","attribute_value_mlt":[{"subitem_description":"Chapter 1: Introduction 1\n1-1. Peptide aptamer 1\n1-2. Disulfide-rich peptides 4\n1-3. In vitro display technologies 7\n1-4. cDNA display 10\n1-5. Aims of this study 14\nChapter 2: Improvement of in vitro selection/evolution system by cDNA display method 15\n2-1. Increasing the library size in cDNA display by optimizing purification procedures 15\n2-1-1. Introduction 15\n2-1-2. Materials and methods 17\n2-1-3. Results and discussion 18\n2-2. A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin-linker. 24\n2-2-1. Introduction 24\n2-2-2. Materials and methods 25\n2-2-3. Results and discussion 28\n2-3. Conclusions 32\nChapter 3: In vitro selection of disulfide-rich peptides against amino groups by cDNA display method 33\n3-1. Introduction 33\n3-2. Materials and methods 35\n3-2-1. Library construction 35\n3-2-2. SBP-linker synthesis 37\n3-2-3. cDNA display preparation from library DNAs 39\n3-2-4. Affinity selection 41\n3-2-5. Peptides 43\n3-2-6. Trypsin digestion and mass spectrometry analysis 44\n3-2-7. Binding assay against amino group modified magnetic beads 44\n3-2-8. Binding assay against a glass slide 45\n3-2-9. Binding assay against agarose beads 45\n3-2-10. Circular dichroism analysis 46\n3-3. Results and discussion 47\n3-3-1. In vitro selection of amino group binding peptides 48\n3-3-2. Binding assay by a pull-down method 49\n3-3-3. Analysis of the relationship between disulfide connectivity and function 50\n3-3-4. Binding assay against amino groups on several kinds of solid supports 54\n3-3-5. Binding assay of CP1 (2SS)-α derivative peptides 55\n3-3-6. Secondary structure analysis 57\n3-4. Conclusions 59\nChapter 4: Overall conclusions 60\nReferences 61\nAcknowledgments 69","subitem_description_type":"Other"}]},"item_113_description_25":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"主指導教員 : 根本直人","subitem_description_type":"Other"}]},"item_113_description_33":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_113_description_34":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_113_dissertation_number_19":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第940号"}]},"item_113_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24561/00010307","subitem_identifier_reg_type":"JaLC"}]},"item_113_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"埼玉大学大学院理工学研究科"}]},"item_113_publisher_12":{"attribute_name":"出版者名（別言語）","attribute_value_mlt":[{"subitem_publisher":"Graduate School of Science and Engineering, Saitama University"}]},"item_113_record_name_8":{"attribute_name":"書誌","attribute_value_mlt":[{"subitem_record_name":"博士論文（埼玉大学大学院理工学研究科（博士後期課程））"}]},"item_113_text_31":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[出版社版]"}]},"item_113_text_36":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"GD0000530"}]},"item_113_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"埼玉大学大学院理工学研究科（博士後期課程）理工学専攻"}]},"item_113_text_5":{"attribute_name":"著者 所属（別言語）","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Science and Engineering, Saitama University"}]},"item_113_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"望月, 佑樹","creatorNameLang":"ja"},{"creatorName":"モチヅキ, ユウキ","creatorNameLang":"ja-Kana"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-23"}],"displaytype":"detail","filename":"GD0000530.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"GD0000530.pdf","objectType":"fulltext","url":"https://sucra.repo.nii.ac.jp/record/10313/files/GD0000530.pdf"},"version_id":"3fd92f26-8c59-4fdf-8b21-9277f4c6164e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"架橋構造を有する分子認識ペプチドの試験管内淘汰に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"架橋構造を有する分子認識ペプチドの試験管内淘汰に関する研究","subitem_title_language":"ja"}]},"item_type_id":"113","owner":"15","path":["504"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-02-04"},"publish_date":"2015-02-04","publish_status":"0","recid":"10313","relation_version_is_last":true,"title":["架橋構造を有する分子認識ペプチドの試験管内淘汰に関する研究"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-06-23T04:43:01.055940+00:00"}