{"created":"2023-05-15T15:23:29.330283+00:00","id":10324,"links":{},"metadata":{"_buckets":{"deposit":"e7fded8f-0c0d-4b45-bbc5-2154d282f669"},"_deposit":{"created_by":15,"id":"10324","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"10324"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00010324","sets":["94:429:431:432:504"]},"author_link":[],"item_113_biblio_info_9":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014","bibliographicIssueDateType":"Issued"}}]},"item_113_date_35":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-02-04","subitem_date_issued_type":"Created"}]},"item_113_date_granted_20":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-24"}]},"item_113_degree_grantor_22":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"埼玉大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"12401","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_113_degree_name_21":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(学術)"}]},"item_113_description_13":{"attribute_name":"形態","attribute_value_mlt":[{"subitem_description":"72p","subitem_description_type":"Other"}]},"item_113_description_24":{"attribute_name":"目次","attribute_value_mlt":[{"subitem_description":"第1章 ..................................................................................................................................... 1\n1.1. はじめに ...................................................................................................................... 2\n1.1.1. カビ臭による被害 ................................................................................................. 2\n1.1.2. 湖沼・貯水池での異臭発生とその防止法 ............................................................. 2\n1.1.3. シアノバクテリアの増殖因子 ............................................................................... 3\n1.1.4. カビ臭原因物質 ..................................................................................................... 4\n1.1.5. ジェオスミン及び2-MIB 産生種.......................................................................... 5\n1.1.6. ジェオスミン合成経路と関連遺伝子の単離 ......................................................... 6\n1.1.7. シアノバクテリアのジェオスミン合成遺伝子 ..................................................... 8\n1.1.8. ジェオスミン遺伝子のモニタリング手法の開発 .................................................. 8\n1.1.9. 放線菌より単離された2-MIB 合成関連遺伝子 .................................................... 9\n1.1.10. GPPMT 及びMIBS の機能解析 ....................................................................... 10\n1.1.11. シアノバクテリアにおけるGPPMT 及びMIBS 遺伝子の単離 ...................... 11\n1.1.12. 遺伝子の発現解析 ............................................................................................. 13\n1.1.13. MIB タンパク質の構造解析 .............................................................................. 13\n1.2. 本論文の概要 ............................................................................................................. 14\n1.2.1. 本論文の目的と意義 ........................................................................................... 14\n1.2.2. 本論文の構成 ...................................................................................................... 15\n第2章 ................................................................................................................................... 17\n2.1. はじめに .................................................................................................................... 18\n2.2. 実験方法 .................................................................................................................... 19\n2.2.1. 使用株と培養条件 ............................................................................................... 19\n2.2.2. DNA 抽出 ............................................................................................................ 21\n2.2.3. 遺伝子の単離 ...................................................................................................... 21\n2.2.4. クローニング ...................................................................................................... 22\n2.2.5. シークエンス ...................................................................................................... 22\n2.2.6. オペロンの解析 ................................................................................................... 23\n2.3. 結果 ............................................................................................................................ 23\n2.3.1. pgmtf 配列の決定 ................................................................................................ 23\n2.3.2. pgmtc 配列の決定 ................................................................................................ 24\n2.3.3 オペロンにおけるpgmtf とpgmtc の配置........................................................... 24\n2.4. 考察 ............................................................................................................................ 25\n第3章 ................................................................................................................................... 36\n3.1. はじめに .................................................................................................................... 37\n3.2. 実験方法 .................................................................................................................... 38\n3.2.1. 培養条件の検討 ................................................................................................... 38\n3.2.2. 2-MIB 濃度の測定 ............................................................................................... 38\n3.2.3. 温度処理実験における培養条件 ......................................................................... 39\n3.2.4. RNA 抽出 ............................................................................................................. 39\n3.2.5. cDNA 合成 ........................................................................................................... 40\n3.2.6. Real-time RT-PCR による発現解析.................................................................... 40\n3.3. 結果 ............................................................................................................................ 41\n3.3.1. P.galeata の増殖に対する温度の影響 ................................................................. 41\n3.3.2. 低温における発現解析 ........................................................................................ 41\n3.3.3. 高温における発現解析 ........................................................................................ 41\n3.4. 考察 ............................................................................................................................ 42\n第4章 ................................................................................................................................... 48\n4.1. はじめに .................................................................................................................... 49\n4.2. 実験方法 .................................................................................................................... 50\n4.2.1. 培養条件 .............................................................................................................. 50\n4.2.2. キャピラリー電気泳動による代謝解析 .............................................................. 50\n4.2.3. データ解析 .......................................................................................................... 50\n4.3. 実験結果 .................................................................................................................... 52\n4.3.1. 化合物の測定 ...................................................................................................... 52\n4.3.2. 主成分分析 .......................................................................................................... 52\n4.3.3. クラスター解析及びヒートマップ ...................................................................... 52\n4.3.4. 培養温度による代謝変動 .................................................................................... 52\n4.3.5. 相関解析 .............................................................................................................. 52\n4.4. 考察 ............................................................................................................................ 53\n第5章 ................................................................................................................................... 60\n5.1. 本研究のまとめ ......................................................................................................... 61\n5.2. 今後の展望 ................................................................................................................. 62\n参考文献 ................................................................................................................................ 63\n活動実績 ................................................................................................................................ 70\n謝辞 ....................................................................................................................................... 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