@article{oai:sucra.repo.nii.ac.jp:00012110,
 author = {Nitta, Koji and 金子, 康子},
 issue = {6},
 journal = {Journal of electron microscopy},
 month = {Dec},
 note = {Simple plunge freezing in liquid propane was applied to plant tissues to observe ultrastructure close to the living state. With young pea leaves, the method yielded well preserved ultrastructure to a depth of up to 40μm from the surface of the specimen. Within the well frozen area, all the membranes appeared smooth and ultrastructural details of each organelle were similar to those obtained by high pressure freezing. Many physical connections between membranes were visualized. The simplicity and satisfactory freezing performance of the method make it suitable for routine ultrastructural studies to capture features of actively functioning cells., This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Journal of Electron Microscopy following peer review. The definitive publisher-authenticated version Oxford University Press, Journal of Electron Microscopy 2004 53(6):677-680; doi:10.1093/jmicro/dfh092 online at: http://jmicro.oxfordjournals.org/cgi/content/abstract/53/6/677, text, application/pdf},
 title = {Simple plunge freezing applied to plant tissues for capturing the ultrastructure close to the living state},
 volume = {53},
 year = {2004},
 yomi = {カネコ, ヤスコ}
}