{"created":"2023-05-15T15:25:20.948109+00:00","id":12945,"links":{},"metadata":{"_buckets":{"deposit":"0434cf1b-5aa9-446b-b3d6-5e992a88d2b7"},"_deposit":{"created_by":3,"id":"12945","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"12945"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00012945","sets":["94:426"]},"author_link":["20436","20438","20437","20435"],"item_119_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"374","bibliographicPageStart":"365","bibliographicVolumeNumber":"3","bibliographic_titles":[{"bibliographic_title":"International Journal of Biological Sciences"}]}]},"item_119_description_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_description":"http://www.biolsci.org/v03p0365.htm | http://www.biolsci.org/v03p0365.htm","subitem_description_type":"Other"}]},"item_119_description_19":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A novel method to link a nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was developed. The mRNA harboring amber stop codon at just downstream of initiation site was hybridized with hydrazide-modified ssDNA at upstream of coding region and was ligated to the DNA. This construct was then modified with 4-acetyl-phenylalanyl amber suppressor tRNA. This modified construct was fused with the nascent protein via the phenylalanine derivative when the mRNA uses the amber suppressor tRNA to decode the amber stop codon. The obtained fusion molecule was used successfully in selective enrichment experiments. It will be applicable for high-through-put screening in evolutionary protein engineering. In contrast to fusion molecules generated by other methods in which the protein is linked to genotype molecule through the C- terminus, our fusion molecule will serve to select a protein for which the C-terminus is essential to be active.","subitem_description_type":"Abstract"}]},"item_119_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_119_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_119_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"Ivyspring International Publisher"}]},"item_119_source_id_14":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14492288","subitem_source_identifier_type":"ISSN"}]},"item_119_text_27":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[著者版]"}]},"item_119_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Ueno, Shingo"},{"subitem_text_value":"Arai, Hidenao"},{"subitem_text_value":"Suzuki, Miho"},{"subitem_text_value":"Husimi, Yuzuru"}]},"item_119_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"A1002279"}]},"item_119_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"埼玉大学理学部細胞制御学研究室"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"埼玉大学大学院理工学研究科物質科学部門物質機能領域"}]},"item_119_text_5":{"attribute_name":"著者 所属(別言語)","attribute_value_mlt":[{"subitem_text_value":"Faculty of Science, Saitama University"},{"subitem_text_value":"Department of Functional Materials Science, Graduate School of Science and Engineering, Saitama University"}]},"item_119_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"2007"}]},"item_119_version_type_28":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"上野, 真吾"},{"creatorName":"ウエノ, シンゴ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arai, Hidenao"},{"creatorName":"アライ, ヒデナオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Suzuki, Miho"},{"creatorName":"スズキ, ミホ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"伏見, 譲"},{"creatorName":"フシミ, ユズル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"A1002279.pdf","filesize":[{"value":"2.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A1002279.pdf","url":"https://sucra.repo.nii.ac.jp/record/12945/files/A1002279.pdf"},"version_id":"eadb3818-15bf-4186-96c5-b19dd1aa9b9d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"In vitro selection","subitem_subject_scheme":"Other"},{"subitem_subject":"mRNA-display","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro virus","subitem_subject_scheme":"Other"},{"subitem_subject":"C-terminus","subitem_subject_scheme":"Other"},{"subitem_subject":"Non-natural amino acid","subitem_subject_scheme":"Other"},{"subitem_subject":"T4 RNA Ligase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering"}]},"item_type_id":"119","owner":"3","path":["426"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-10-17"},"publish_date":"2007-10-17","publish_status":"0","recid":"12945","relation_version_is_last":true,"title":["An mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-16T09:23:16.762324+00:00"}