@article{oai:sucra.repo.nii.ac.jp:00013142,
 author = {Saitoh, Kazunori and Yamada, Noriko and Ishikawa, Eri and Nakajima, Hizuru and 渋川, 雅美},
 issue = {1},
 journal = {Journal of Separation Science},
 month = {},
 note = {A new on-line redox derivatization technique using double separation columns and one redox derivatization unit was presented for enhancement of separation selectivity of HPLC. This on-line redox derivatization HPLC system consisted of two separation columns and one redox derivatization unit placed between them. The redox reaction proceeds in the derivatization unit so that an analyte compound migrates as its original form in the first column, while as its oxidized or reduced form in the second column. The retention of the analytes is controlled by the lengths of the two separation columns in this system. We adopted a small column packed with porous graphitic carbon (PGC) as a redox derivatization unit and two C-18 silica columns treated with hexadecyltrimethylammonium. chloride as separation columns. The redox activity of PGC and the efficiency of the on-line redox derivatization HPLC system for enhancement of separation selectivity were investigated using EDTA complexes of some metal ions. Original untreated PGC and PGC treated with hydrogen peroxide completely oxidized Co(II)-EDTA and converted it to Co(Ill)-EDTA, while the other metal complexes eluted as their original oxidation states throughout the system. Selective separation and determination of cobalt in a reference copper alloy by the developed method were demonstrated., Copyright(c)2006 John Wiley & Sons Ltd, text, application/pdf},
 pages = {49--56},
 title = {On-line redox derivatization liquid chromatography using double separation columns and one derivatization unit},
 volume = {29},
 year = {2006},
 yomi = {シブカワ, マサミ}
}