@article{oai:sucra.repo.nii.ac.jp:00013147,
 author = {石井, 一行 and 田中, 由香 and 秦, 恭子 and 後藤, 将治 and 齊藤, 和憲 and 南澤, 宏明 and 渋川, 雅美},
 issue = {9},
 journal = {分析化学},
 month = {},
 note = {本研究では,水性二相系高速向流クロマトグラフィー(HSCCC)による無機化合物の分離を目的として,操作条件と分離性能の関係を検討した.HSCCC 装置としてはJ型コイルプラネット向流クロマトグラフ,水性二相系としてはポリエチレングリコール(PEG)-Na_2SO_4系を用い,無機陰イオン (IO_3^-,I^-,NO_3^-,SCN^-)をモデル化合物として,二相系の組成,ドラムの回転速度及び移動相流量と分離度の関係を検討した.その結果,カラムを巻き付けたドラムの回転速度が大きいほど固定相保持百分率が減少したが,それにもかかわらず分離度は大きくなるという現象が観測された. 理論段数を測定したところ,分離度の増加はほぼ理論段数の増加で説朋できることが明らかになった.これは,回転速度の増加に伴って二相のかくはんが激しくなり,液滴が小さくなることによるものと推測される.一方,移動相流量は分離効率に大きな影響を与えなかった.得られた結果に基づいて最適条件を設定し, Cr(III)とCr(VI)の分離を試みたところ良好な分離結果が得られた.
The optimum experimental conditions for J coil planet centrifuge high-speed countercurrent chromatography (HSCCC) with aqueous biphasic systems consisting of polyethylene glycol (PEG) and sodium sulfate were investigated using some inorganic anions as model compounds. The PEG-rich upper phase was used as the stationary phase and the salt-rich lower phase was used as the mobile phase. In order to eliminate fluctuations of the base line of chromatograms owing to emulsification caused by excessive mixing of the two phases, water was mixed with the eluate from the column prior to UV detection. The resolution of iodate ion and iodide ion increased with an increase in the revolution speed of the Teflon tube column holder although the volume of the stationary phase retained in the column decreased. This was due to an improvement of the separation efficiency, shown as an increase in theoretical number of sample compounds. The increase in the flow rate of the mobile phase caused a decrease in the stationary phase volume. However, the resolution did not depend on the flow rate in the range studied. This may be interpreted in terms of relatively high distribution ratios of the inorganic anions used in this study. Separation of some inorganic anions and that of chromium(III) ion and chromate(VI) ion were easily accomplished by HSCCC with PEG, text, application/pdf},
 pages = {911--917},
 title = {水性二相系高速向流クロマトグラフィーによる無機イオンの分離},
 volume = {53},
 year = {2004},
 yomi = {イシイ, カズユキ and タナカ, ユカ and ハタ, キョウコ and ゴトウ, マサハル and サトウ, カズノリ and ミナミサワ, ヒロアキ and シブカワ, マサミ}
}