@article{oai:sucra.repo.nii.ac.jp:00013335,
 author = {Kimpara, Tomoya and Aohara, Tsutomu and Soga, Kouichi and Wakabayashi, Kazuyuki and Hoson, Takayuki and Tsumuraya, Yoichi and 小竹, 敬久},
 issue = {2},
 journal = {ANNALS OF BOTANY},
 month = {},
 note = {Background and Aims The metabolism of beta-1,3 : 1,4-glucan regulates the mechanical properties of cell walls, and thereby changes the elongation growth of Poaceae plants. A previous study has shown that elongation growth of rice coleoptiles under water is enhanced by increased activity of beta-1,3 : 1,4-glucan hydrolases; however, the involvement of beta-1,3 : 1,4-glucan synthase activity in elongation growth under water has not yet been clarified.
Methods The beta-1,3 : 1,4-glucan synthase activity in a microsomal fraction prepared from rice seedlings grown under water was compared with that from control seedlings grown in air. The change under water in the relative expression level of CslF6, a major isoform of the beta-1,3 : 1,4-glucan synthase genes, was examined by quantitative reverse-transcriptase PCR.
Key Results The level of beta-1,3 : 1,4-glucan synthase activity in submerged seedlings decreased to less than 40% of that of the control seedlings and was accompanied by a significant reduction in the amount of beta-1,3 : 1,4-glucan in the cell walls. Under water, the expression of CslF6 was reduced to less than 20% of the unsubmerged control. Bubble aeration partially restored both beta-1,3 : 1,4-glucan synthase activity and the expression of CslF6 under water, correlating with suppression of the submergence-induced elongation growth of coleoptiles.
Conclusions Submergence down-regulates the expression of the CslF6 gene, leading to a decreased level of beta-1,3 : 1,4-glucan synthase activity. Together with the increased activity of beta-1,3 : 1,4-glucan hydrolases, the decreased activity of beta-1,3 : 1,4-glucan synthase contributes to the decrease in the amount of beta-1,3 : 1,4-glucan in the cell walls underwater. The suppression of beta-1,3 : 1,4-glucan synthesis under water may be mainly due to oxygen depletion., text, application/pdf},
 pages = {221--226},
 title = {beta-1,3 : 1,4-glucan synthase activity in rice seedlings under water},
 volume = {102},
 year = {2008},
 yomi = {コタケ, トシヒサ}
}