{"created":"2023-05-15T15:25:53.358839+00:00","id":13684,"links":{},"metadata":{"_buckets":{"deposit":"f5dd05dd-e595-45b6-b34c-6e05d08cd713"},"_deposit":{"created_by":3,"id":"13684","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13684"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00013684","sets":["94:426"]},"author_link":["22827","22826","22825"],"item_119_alternative_title_1":{"attribute_name":"タイトル（別言語）","attribute_value_mlt":[{"subitem_alternative_title":"Effect of Pencycuron on the Osmotic Stability of Protoplasts of Rhizoctonia solani"}]},"item_119_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"163","bibliographicPageStart":"159","bibliographicVolumeNumber":"21","bibliographic_titles":[{"bibliographic_title":"日本農薬学会誌"},{"bibliographic_title":"Journal of pesticide science","bibliographic_titleLang":"en"}]}]},"item_119_date_31":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-10-08","subitem_date_issued_type":"Created"}]},"item_119_description_19":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ペンシクロンはきわめて作用特異性の高い薬剤で, Rhizoctonia solaniの同一菌糸融合群(AG4)に対してもその作用に顕著な差異が認められる.菌糸融合群4に属するR-C株(ペンシクロン感受性)およびRh-131株(同薬剤非感受性)を選び, 病原菌の細胞膜強度に及ぼすペンシクロンの影響を検討したところ, R-Cではプロトプラストからのコロニー再生率が薬剤存在下での低浸透圧処理により対照区(低浸透圧処理のみ)に比べて有意に減少した.しかし, 薬剤を洗浄後, 低浸透圧処理すると対照区と同等なコロニーの再生率を示した.一方, Rh-131ではプロトプラストからの再生率は低浸透圧処理により減少するが, ペンシクロン存在下におけるコロニーの形成は逆に促進されるような結果が得られた.ペンシクロン処理直後の短時間の間の薬剤の細胞膜に及ぼす影響をプロトプラスト懸濁液の吸光度の変化を測定して調べたところ, R-Cのプロトプラスト懸濁液に薬剤存在下で低浸透圧処理すると懸濁液の吸光度は顕著に減少したが, Rh-131のプロトプラスト懸濁液での低浸透圧処理の効果はペンシクロンの存在によって有意な影響を受けなかった.これらの結果からペンシクロンは感受性菌の細胞膜に特異的に影響を与えることが示唆された.\n\nPencycuron shows a specific inhibitory activity on the mycelial growth of Rhizoctonia solani. Even in the same anastomosis groups (Ags), e.g. AG4, there are both isolates sensitive and less sensitive (inherently resistant) to pencycuron. The regeneration of colonies from protoplasts of R-C (pencycuron-sensitive isolate in AG4) significantly decreased by the osmotic shock in the presence of pencycuron, while such effect was cancelled by washing off the chemical prior to the osmotic shock. However, in Rh-131 (a less sensitive isolate in AG4), the application of the chemical appeared to stimulate the regeneration from protoplasts. Further the measurement of optical density of protoplasts suspensions was performed to elucidate the effect of pencycuron on the osmotic stability of protoplasts in a short period. The optical density of suspensions of R-C protoplasts rapidly declined in a short period of incubation by the osmotic shock in the presence of pencycuron, but the effect on Rh-131 protoplasts was not statistically significant regardless of the presence or the absence of the chemical. These results suggest that the cell membrane of the pencycuron-sensitive isolate (R-C) is specifically affected by pencycuron.","subitem_description_type":"Abstract"}]},"item_119_description_21":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"rights: 日本農薬学会\nrights: 本文データは学協会の許諾に基づきCiNiiから複製したものである\nrelation: IsVersionOf: http://ci.nii.ac.jp/naid/110001712907/","subitem_description_type":"Other"}]},"item_119_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_119_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_119_other_language_22":{"attribute_name":"その他の言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_119_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"日本農薬学会"}]},"item_119_source_id_14":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03851559","subitem_source_identifier_type":"ISSN"}]},"item_119_text_27":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[出版社版]"}]},"item_119_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"KIM Heung, Tae"},{"subitem_text_value":"KAMAKURA, Takashi"},{"subitem_text_value":"YAMAGUCHI, Isamu"}]},"item_119_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"A1003665"}]},"item_119_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"埼玉大学大学院理工学研究科"}]},"item_119_text_5":{"attribute_name":"著者 所属(別言語)","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Science and Engineering, Saitama University"},{"subitem_text_value":"The Institute of Physical and Chemical Research (RIKEN)"},{"subitem_text_value":"The Institute of Physical and Chemical Research (RIKEN)"}]},"item_119_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"1996-5"}]},"item_119_version_type_28":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"金, 興泰"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鎌倉, 高志"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山口, 勇"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"A1003665.pdf","filesize":[{"value":"528.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A1003665.pdf","url":"https://sucra.repo.nii.ac.jp/record/13684/files/A1003665.pdf"},"version_id":"d3a79fa9-f604-43cf-a732-a8fbebe58d6e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Rhizoctonia solani のプロトプラストの浸透圧耐性に及ぼすペンシクロンの影響","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Rhizoctonia solani のプロトプラストの浸透圧耐性に及ぼすペンシクロンの影響"}]},"item_type_id":"119","owner":"3","path":["426"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-10-19"},"publish_date":"2009-10-19","publish_status":"0","recid":"13684","relation_version_is_last":true,"title":["Rhizoctonia solani のプロトプラストの浸透圧耐性に及ぼすペンシクロンの影響"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T19:09:58.276539+00:00"}