{"created":"2023-05-15T15:25:57.372495+00:00","id":13779,"links":{},"metadata":{"_buckets":{"deposit":"fc182c37-c636-40c8-b400-15ad70ecbc87"},"_deposit":{"created_by":3,"id":"13779","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13779"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00013779","sets":["94:426"]},"author_link":["23043","23038","23040","23042","23039","23041","17870"],"item_119_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"28","bibliographicPageEnd":"18691","bibliographicPageStart":"18685","bibliographicVolumeNumber":"284","bibliographic_titles":[{"bibliographic_title":"JOURNAL OF BIOLOGICAL CHEMISTRY"}]}]},"item_119_date_31":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-08-20","subitem_date_issued_type":"Created"}]},"item_119_description_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_description":"http://www.jbc.org/cgi/content/abstract/284/28/18685 | http://www.jbc.org/cgi/content/abstract/284/28/18685","subitem_description_type":"Other"}]},"item_119_description_19":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Elongation factor G (EF-G), a key protein in translational elongation, was identified as a primary target of inactivation by reactive oxygen species within the translational machinery of the cyanobacterium Synechocystis sp. PCC 6803 (Kojima, K., Oshita, M., Nanjo, Y., Kasai, K., Tozawa, Y., Hayashi, H., and Nishiyama, Y. (2007) Mol. Microbiol. 65, 936–947). In the present study, we found that inactivation of EF-G (Slr1463) byH2O2 was attributable to the oxidation of two specific cysteine residues and formation of a disulfide bond. Substitution of these cysteine residues by serine residues protected EF-G from inactivation byH2O2 and allowed the EF-G to mediate translation in a translation system in vitro that had been prepared from Synechocystis.\nThe disulfide bond in oxidized EF-G was reduced by thioredoxin, and the resultant reduced form of EF-G regained the activity to mediate translation in vitro. Western blotting analysis showed that levels of the oxidized form of EF-G increased under strong light in a mutant that lacked NADPHthioredoxin reductase, indicating that EF-G is reduced by thioredoxin in vivo. These observations suggest that the translational machinery is regulated by the redox state of EF-G, which is oxidized by reactive oxygen species and reduced by thioredoxin, a transmitter of reducing signals generated by the photosynthetic transport of electrons.","subitem_description_type":"Abstract"}]},"item_119_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_119_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_119_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology"}]},"item_119_relation_16":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1074/jbc.M109.015131","subitem_relation_type_select":"DOI"}}]},"item_119_source_id_14":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00219258","subitem_source_identifier_type":"ISSN"}]},"item_119_text_27":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[出版社版]"}]},"item_119_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Kojima, Kouji"},{"subitem_text_value":"Motohashi, Ken"},{"subitem_text_value":"Morota, Takuya"},{"subitem_text_value":"Oshita, Masaru"},{"subitem_text_value":"Hisabori, Toru"},{"subitem_text_value":"Hayashi, Hidenori"},{"subitem_text_value":"Nishiyama, Yoshitaka"}]},"item_119_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"A1002932"}]},"item_119_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"埼玉大学大学院理工学研究科生命科学部門"}]},"item_119_text_5":{"attribute_name":"著者 所属(別言語)","attribute_value_mlt":[{"subitem_text_value":"Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University"},{"subitem_text_value":"Chemical Resources Laboratory, Tokyo Institute of Technology"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"Department of Biochemistry and Molecular Biology, Graduate School of Science"}]},"item_119_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"2009-7"}]},"item_119_version_type_28":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kojima, Kouji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Motohashi, Ken"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Morota, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oshita, Masaru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hisabori, Toru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hayashi, Hidenori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西山, 佳孝"},{"creatorName":"ニシヤマ, ヨシタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"A1002932.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A1002932.pdf","url":"https://sucra.repo.nii.ac.jp/record/13779/files/A1002932.pdf"},"version_id":"a9017f3c-98c1-422e-8214-7505e6c6cb6c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Regulation of Translation by the Redox State of Elongation Factor G in the Cyanobacterium Synechocystis sp PCC 6803","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulation of Translation by the Redox State of Elongation Factor G in the Cyanobacterium Synechocystis sp PCC 6803"}]},"item_type_id":"119","owner":"3","path":["426"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-08-20"},"publish_date":"2010-08-20","publish_status":"0","recid":"13779","relation_version_is_last":true,"title":["Regulation of Translation by the Redox State of Elongation Factor G in the Cyanobacterium Synechocystis sp PCC 6803"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-16T09:27:04.253338+00:00"}