{"created":"2023-05-15T15:26:00.050371+00:00","id":13840,"links":{},"metadata":{"_buckets":{"deposit":"e4a3d46b-39c0-4ea9-96b9-c3abb5d6df2f"},"_deposit":{"created_by":3,"id":"13840","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13840"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00013840","sets":["96:641"]},"author_link":["28642","23238","23237","23236","23239"],"item_119_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"449","bibliographicPageStart":"443","bibliographicVolumeNumber":"110","bibliographic_titles":[{"bibliographic_title":"JOURNAL OF BIOCHEMISTRY"}]}]},"item_119_date_31":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2008-08-20","subitem_date_issued_type":"Created"}]},"item_119_description_19":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A simple, specific, and sensitive assay procedure for cardiolipin synthase of Escherichia coli has been developed. This measures the radioactivity of glycerol formed from phosphatidyl[2-3H]glycerol and is mainly based on the findings that 400 mM phosphate and 0.015% Triton X-100 markedly activate the enzyme. Cardiolipin synthase was amplified 760-fold upon induction with isopropyl β-D-thiogalactoside in cells harboring a pBR322 derivative in which the cls gene encoding this enzyme was preceded by the tac promoter. Under these conditions, cardiolipin content increased, membrane potential decreased, spheroplasts became fragile, cells lost viability, and inducer-resistant mutants appeared at a high frequency. The amplification enabled the isolation of an enzyme preparation with a specific activity approximately 10,000-times higher than that of wild-type whole cell lysate. This purification was simply achieved by extraction of the crude membrane fraction with Triton X-100 and a single phosphocellulose column chromatography. This preparation, together with the crude envelope fraction, was used to characterize the basic properties of E. coli cardiolipin synthase, some of which were utilized in setting up the assay conditions.","subitem_description_type":"Abstract"}]},"item_119_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_119_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_119_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"Japanese Biochemical Society"}]},"item_119_source_id_14":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021924X","subitem_source_identifier_type":"ISSN"}]},"item_119_text_27":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[出版社版]"}]},"item_119_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Hiraoka, Shuichi"},{"subitem_text_value":"Nukui, Kazuki"},{"subitem_text_value":"Uetake, Nobuyuki"},{"subitem_text_value":"Ohta, Akinori"},{"subitem_text_value":"Shibuya, Isao"}]},"item_119_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"A1002763"}]},"item_119_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"埼玉大学理学部(現 : 東京大学大学院農学生命科学研究科応用生命工学専攻細胞遺伝研究室)"}]},"item_119_text_5":{"attribute_name":"著者 所属(別言語)","attribute_value_mlt":[{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"Department of Biochemistry, Saitama University(Present : Department of Biotechnology, The University of Tokyo)"}]},"item_119_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"1991-9"}]},"item_119_version_type_28":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hiraoka, Shuichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nukui, Kazuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uetake, Nobuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"太田, 明徳"},{"creatorName":"オオタ, アキノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibuya, Isao"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"A1002763.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A1002763.pdf","url":"https://sucra.repo.nii.ac.jp/record/13840/files/A1002763.pdf"},"version_id":"56902ba2-e883-4706-9a30-f0f4ca7f7987"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"AMPLIFICATION AND SUBSTANTIAL PURIFICATION OF CARDIOLIPIN SYNTHASE OF ESCHERICHIA-COLI","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"AMPLIFICATION AND SUBSTANTIAL PURIFICATION OF CARDIOLIPIN SYNTHASE OF ESCHERICHIA-COLI"}]},"item_type_id":"119","owner":"3","path":["641"],"pubdate":{"attribute_name":"公開日","attribute_value":"2008-08-19"},"publish_date":"2008-08-19","publish_status":"0","recid":"13840","relation_version_is_last":true,"title":["AMPLIFICATION AND SUBSTANTIAL PURIFICATION OF CARDIOLIPIN SYNTHASE OF ESCHERICHIA-COLI"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T19:44:09.829487+00:00"}