{"created":"2023-05-15T15:26:13.306498+00:00","id":14149,"links":{},"metadata":{"_buckets":{"deposit":"1f7f3de6-b1d8-4d64-958b-76b4d7fcaab3"},"_deposit":{"created_by":3,"id":"14149","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14149"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00014149","sets":["96:641"]},"author_link":["24197","24196"],"item_119_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2005","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"96","bibliographicPageStart":"92","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"Microbes and environments"}]}]},"item_119_date_31":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-10-08","subitem_date_issued_type":"Created"}]},"item_119_description_19":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A gene for cAMP phosphodiesterase, designated cpdA, was identified in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120. The predicted amino acid sequence of the gene was similar to the sequences of cAMP phosphodiesterases from Thermosynechococcus elongatus, Escherichia coli and Haemophilus influenzae. The recombinant protein was purified by sequential column chromatography and its biochemical properties were determined. The Anabaena cAMP phosphodiesterase hydrolyzed cAMP and cGMP with similar levels of activity. The K_m value for cAMP was 45μM and the Vmax was 4.9μmol min^<-1> mg protein^<-1>. These values are similar to those of Escherichia coli cAMP phosphodiesterase. The enzyme was activated by divalent cations such as Fe^<2+> and Mn^<2+>. The tertiary structure of this enzyme was predicted by homology modeling. The deduced structure has two metal-binding sites in the catalytic domain.","subitem_description_type":"Abstract"}]},"item_119_description_21":{"attribute_name":"注記","attribute_value_mlt":[{"subitem_description":"rights: 日本微生物生態学会\nrights: 本文データは学協会の許諾に基づきCiNiiから複製したものである\nrelation: IsVersionOf: http://ci.nii.ac.jp/naid/110001282480/","subitem_description_type":"Other"}]},"item_119_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_119_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_119_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"日本微生物生態学会"}]},"item_119_relation_16":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1264/jsme2.20.92","subitem_relation_type_select":"DOI"}}]},"item_119_source_id_14":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13426311","subitem_source_identifier_type":"ISSN"}]},"item_119_text_27":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[出版社版]"}]},"item_119_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"FUJISAWA, TAKATOMO"},{"subitem_text_value":"OHMORI, MASAYUKI"}]},"item_119_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"A1003671"}]},"item_119_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"東邦大学 ; 埼玉大学理学部"}]},"item_119_text_5":{"attribute_name":"著者 所属(別言語)","attribute_value_mlt":[{"subitem_text_value":"Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo"},{"subitem_text_value":"Center of Systems Biology and Ecology, Faculty of Science, Toho University ; (Present address)Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University"}]},"item_119_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"2005-6"}]},"item_119_version_type_28":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"FUJISAWA, TAKATOMO"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"OHMORI, MASAYUKI"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"A1003671.pdf","filesize":[{"value":"562.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A1003671.pdf","url":"https://sucra.repo.nii.ac.jp/record/14149/files/A1003671.pdf"},"version_id":"fe8d39bf-711b-4482-9edd-578dc094bf88"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cyclic nucleotide phosphodiesterase","subitem_subject_scheme":"Other"},{"subitem_subject":"cAMP signal transduction","subitem_subject_scheme":"Other"},{"subitem_subject":"cyanobacteria","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biochemical Properties of a cAMP Phosphodiesterase in the Cyanobacterium Anabaena sp. strain PCC 7120","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biochemical Properties of a cAMP Phosphodiesterase in the Cyanobacterium Anabaena sp. strain PCC 7120"}]},"item_type_id":"119","owner":"3","path":["641"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-10-20"},"publish_date":"2009-10-20","publish_status":"0","recid":"14149","relation_version_is_last":true,"title":["Biochemical Properties of a cAMP Phosphodiesterase in the Cyanobacterium Anabaena sp. strain PCC 7120"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T12:09:47.103903+00:00"}