@article{oai:sucra.repo.nii.ac.jp:00014475,
 author = {大倉, 正道 and SASAKI, Takuya and SADAKARI, Junko and 安藤, 恵子 and KAGAWA-NAGAMURA, Yuko and KOBAYASHI, Chiaki and IKEGAYA, Yuji and 中井, 淳一},
 issue = {12},
 journal = {PLoS ONE},
 month = {},
 note = {e51286, Imaging the activities of individual neurons with genetically encoded Ca2+ indicators (GECIs) is a promising method for understanding neuronal network functions. Here, we report GECIs with improved neuronal Ca2+ signal detectability, termed G-CaMP6 and G-CaMP8. Compared to a series of existing G-CaMPs, G-CaMP6 showed fairly high sensitivity and rapid kinetics, both of which are suitable properties for detecting subtle and fast neuronal activities. G-CaMP8 showed a greater signal (Fmax/Fmin = 38) than G-CaMP6 and demonstrated kinetics similar to those of G-CaMP6. Both GECIs could detect individual spikes from pyramidal neurons of cultured hippocampal slices or acute cortical slices with 100% detection rates, demonstrating their superior performance to existing GECIs. Because G-CaMP6 showed a higher sensitivity and brighter baseline fluorescence than G-CaMP8 in a cellular environment, we applied G-CaMP6 for Ca2+ imaging of dendritic spines, the putative postsynaptic sites. By expressing a G-CaMP6-actin fusion protein for the spines in hippocampal CA3 pyramidal neurons and electrically stimulating the granule cells of the dentate gyrus, which innervate CA3 pyramidal neurons, we found that sub-threshold stimulation triggered small Ca2+ responses in a limited number of spines with a low response rate in active spines, whereas supra-threshold stimulation triggered large fluorescence responses in virtually all of the spines with a 100% activity rate., text, application/pdf},
 pages = {1--10},
 title = {Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals},
 volume = {7},
 year = {2012},
 yomi = {オオクラ, マサミチ and ササキ, タクヤ and サダカリ, ジュンコ and ゲンギョウ-アンドウ, ケイコ and カガワ-ナガムラ, ユウコ and コバヤシ, チアキ and イケガヤ, ユウジ and ナカイ, ジュンイチ}
}