{"created":"2023-05-15T15:26:28.061065+00:00","id":14475,"links":{},"metadata":{"_buckets":{"deposit":"ed3bcae2-c90f-4a2f-ae64-6d45afcbbfbd"},"_deposit":{"created_by":3,"id":"14475","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14475"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00014475","sets":["95:242"]},"author_link":["17846","24849","16759","24848","24851","24850","16758","24852"],"item_119_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"10","bibliographicPageStart":"1","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_119_date_31":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2015-03-06","subitem_date_issued_type":"Created"}]},"item_119_description_18":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"e51286","subitem_description_type":"Other"}]},"item_119_description_19":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Imaging the activities of individual neurons with genetically encoded Ca2+ indicators (GECIs) is a promising method for understanding neuronal network functions. Here, we report GECIs with improved neuronal Ca2+ signal detectability, termed G-CaMP6 and G-CaMP8. Compared to a series of existing G-CaMPs, G-CaMP6 showed fairly high sensitivity and rapid kinetics, both of which are suitable properties for detecting subtle and fast neuronal activities. G-CaMP8 showed a greater signal (Fmax/Fmin = 38) than G-CaMP6 and demonstrated kinetics similar to those of G-CaMP6. Both GECIs could detect individual spikes from pyramidal neurons of cultured hippocampal slices or acute cortical slices with 100% detection rates, demonstrating their superior performance to existing GECIs. Because G-CaMP6 showed a higher sensitivity and brighter baseline fluorescence than G-CaMP8 in a cellular environment, we applied G-CaMP6 for Ca2+ imaging of dendritic spines, the putative postsynaptic sites. By expressing a G-CaMP6-actin fusion protein for the spines in hippocampal CA3 pyramidal neurons and electrically stimulating the granule cells of the dentate gyrus, which innervate CA3 pyramidal neurons, we found that sub-threshold stimulation triggered small Ca2+ responses in a limited number of spines with a low response rate in active spines, whereas supra-threshold stimulation triggered large fluorescence responses in virtually all of the spines with a 100% activity rate.","subitem_description_type":"Abstract"}]},"item_119_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_119_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_119_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science (PLoS)"}]},"item_119_relation_16":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1371/journal.pone.0051286","subitem_relation_type_select":"DOI"}}]},"item_119_text_27":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[出版社版]"}]},"item_119_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"OHKURA, Masamichi"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"GENGYO-ANDO, Keiko"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"NAKAI, Junichi"}]},"item_119_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"A3000387"}]},"item_119_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"Brain Science Institute, Saitama University"},{"subitem_text_value":"Brain Science Institute, Saitama University"},{"subitem_text_value":"Brain Science Institute, Saitama University"},{"subitem_text_value":"Brain Science Institute, Saitama University"},{"subitem_text_value":"Brain Science Institute, Saitama University"},{"subitem_text_value":"Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, University of Tokyo"},{"subitem_text_value":"Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, University of Tokyo"},{"subitem_text_value":"Brain Science Institute, Saitama University"}]},"item_119_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"2012-12"}]},"item_119_version_type_28":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大倉, 正道"},{"creatorName":"オオクラ, マサミチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SASAKI, Takuya"},{"creatorName":"ササキ, タクヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"SADAKARI, Junko"},{"creatorName":"サダカリ, ジュンコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安藤, 恵子"},{"creatorName":"ゲンギョウ-アンドウ, ケイコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"KAGAWA-NAGAMURA, Yuko"},{"creatorName":"カガワ-ナガムラ, ユウコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"KOBAYASHI, Chiaki"},{"creatorName":"コバヤシ, チアキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"IKEGAYA, Yuji"},{"creatorName":"イケガヤ, ユウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中井, 淳一"},{"creatorName":"ナカイ, ジュンイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"A3000387.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"A3000387.pdf","url":"https://sucra.repo.nii.ac.jp/record/14475/files/A3000387.pdf"},"version_id":"766adda3-4509-40f7-bfdf-640e71985f17"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals"}]},"item_type_id":"119","owner":"3","path":["242"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-09"},"publish_date":"2015-03-09","publish_status":"0","recid":"14475","relation_version_is_last":true,"title":["Genetically Encoded Green Fluorescent Ca2+ Indicators with Improved Detectability for Neuronal Ca2+ Signals"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T11:56:47.391178+00:00"}