@article{oai:sucra.repo.nii.ac.jp:00014504,
 author = {金子, 康子},
 journal = {CACS FORUM},
 month = {},
 note = {Novel three dimensional images were obtained from rapidly frozen plant tissues and cells with a combination of the Alto 1000 cryo system and a low vacuum scanning electron microscope (Hitachi S-3400N). Segments of plant tissue were frozen in slush nitrogen and set in the cryo system. The sample was then fractured with a cooled knife and placed onto the specimen stage in the SEM. After appropriately subliming ice, a back scattered electron (BSE) image was obtained in low vacuum mode. While the fine structure of the cell surface could be observed with an accelerating voltage of 5 kV, intracellular ultrastructure was clearly visible through the cell wall at an accelerating voltage of 15 kV. The method allows the study of internal ultrastructure of plant cells and function close to the living state without lengthy specimen preparation process., text, application/pdf},
 pages = {27--28},
 title = {低温・低真空走査電子顕微鏡による植物組織・細胞の観察《マイレビュー》},
 volume = {1},
 year = {2010},
 yomi = {カネコ, ヤスコ}
}