{"created":"2023-05-15T15:26:40.257677+00:00","id":14765,"links":{},"metadata":{"_buckets":{"deposit":"36f9c27a-8b44-403c-b2a1-8c95d1b609fa"},"_deposit":{"created_by":3,"id":"14765","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14765"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00014765","sets":["95:240:496:484"]},"author_link":["25056"],"item_120_alternative_title_1":{"attribute_name":"タイトル（別言語）","attribute_value_mlt":[{"subitem_alternative_title":"Enzymatic Synthesis of Oligonucleotides and Their Application"}]},"item_120_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"27","bibliographicPageStart":"25","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"CACS FORUM"}]}]},"item_120_date_31":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2010-01-14","subitem_date_issued_type":"Created"}]},"item_120_description_19":{"attribute_name":"概要","attribute_value_mlt":[{"subitem_description":"A new rapid method to synthesize an oligonucleotide 9-mer of any base sequence is proposed. According to the base sequence designed for the oligomer, a 5'tetramer and a 3'tetramer are picked up from a library of tetradeoxyribonucleotides, which have been previously prepared using a DNA synthesizer. The 5'tetramer is added with a mono-ribo-nucleotide (according to the 5th base of the desired sequence) using the terminal deoxynucleotidyl transferase and the 3'tetramer is phosphorylated at 5'terminus using the T4 polynucleotide kinase. Then, the 5'pentamer and the 3'tetramer are combined with the T4 RNA ligase, resulting in a 9-mer having the desired sequence. The 9-mer is proved to serve as a primer for a DNA polymerase reaction without purification steps. This procedure which requires only 2h, can rapidly provide primers for some method of the large-scale DNAsequencmg.","subitem_description_type":"Other"}]},"item_120_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_120_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_120_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24561/00014759","subitem_identifier_reg_type":"JaLC"}]},"item_120_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"埼玉大学分析センター"}]},"item_120_text_27":{"attribute_name":"版","attribute_value_mlt":[{"subitem_text_value":"[出版社版]"}]},"item_120_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"KINOSHITA, Yasunori"}]},"item_120_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"KY-AN10402595-11-06"}]},"item_120_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"埼玉大学工学部環境化学工学科"}]},"item_120_text_5":{"attribute_name":"著者 所属(別言語)","attribute_value_mlt":[{"subitem_text_value":"Department of Environmental Chemistry, Faculty of Engineering., Saitama University"}]},"item_120_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"1991-11"}]},"item_120_version_type_28":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木下, 保則"},{"creatorName":"キノシタ, ヤスノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"KY-AN10402595-11-06.pdf","filesize":[{"value":"167.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KY-AN10402595-11-06.pdf","url":"https://sucra.repo.nii.ac.jp/record/14765/files/KY-AN10402595-11-06.pdf"},"version_id":"f419c66e-51ee-438c-a355-960c96226de3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"酵素を用いたオリゴヌクレオチドの合成と応用<ミニノート>","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酵素を用いたオリゴヌクレオチドの合成と応用<ミニノート>"}]},"item_type_id":"120","owner":"3","path":["484"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-11-12"},"publish_date":"2009-11-12","publish_status":"0","recid":"14765","relation_version_is_last":true,"title":["酵素を用いたオリゴヌクレオチドの合成と応用<ミニノート>"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-15T17:53:29.971606+00:00"}