{"created":"2023-05-15T15:27:43.328774+00:00","id":16640,"links":{},"metadata":{"_buckets":{"deposit":"a690f657-ffb0-46fb-a28d-3d5345164fe8"},"_deposit":{"created_by":3,"id":"16640","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"16640"},"status":"published"},"_oai":{"id":"oai:sucra.repo.nii.ac.jp:00016640","sets":["95:239:634"]},"author_link":["27125","27124","27122","27126","27123","17549"],"item_120_alternative_title_1":{"attribute_name":"タイトル（別言語）","attribute_value_mlt":[{"subitem_alternative_title":"Construction and Application of Peptide-Library Generated by YLBS"}]},"item_120_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"134","bibliographicPageStart":"133","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"埼玉大学地域共同研究センター紀要"},{"bibliographic_title":"Report of Cooperative Research Center, Saitama University","bibliographic_titleLang":"en"}]}]},"item_120_date_31":{"attribute_name":"作成日","attribute_value_mlt":[{"subitem_date_issued_datetime":"2009-06-24","subitem_date_issued_type":"Created"}]},"item_120_description_19":{"attribute_name":"概要","attribute_value_mlt":[{"subitem_description":"Peptides have a big potential to be used for biologically active molecules such as hormone, antibiotics, inhibitors and others. Therefore, they are one of the most important substances targeted by Evolutionary Molecular Engineering. The possibility of building peptide-coding DNA libraries, beginning from 20 species of amino acid-monomer blocks, by use of YLBS technology was examined. Three/ four steps of Y-ligation were performed, providing y3 N 4 DNA libraries corresponding to peptides of 8-mers/16-mers, respectively. Y3 was confirmed to be really successful in containing the whole set of 8mers. Tenacious problems of deletion and uneven appearance of blocks were challenged and fruitfully solved by introducing an operation which does not depend on the second-strand cutting of DNA by restriction enzymes or by starting with preferential blocks for T4 RNA ligation.","subitem_description_type":"Other"}]},"item_120_description_29":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"text","subitem_description_type":"Other"}]},"item_120_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_120_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24561/00016634","subitem_identifier_reg_type":"JaLC"}]},"item_120_other_language_22":{"attribute_name":"その他の言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_120_publisher_11":{"attribute_name":"出版者名","attribute_value_mlt":[{"subitem_publisher":"埼玉大学地域共同研究センター"}]},"item_120_source_id_14":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13474758","subitem_source_identifier_type":"ISSN"}]},"item_120_text_3":{"attribute_name":"著者 ローマ字","attribute_value_mlt":[{"subitem_text_value":"Kobayashi, Teruaki"},{"subitem_text_value":"Narasaki, Shinsuke"},{"subitem_text_value":"Kinoshita, Yasunori"},{"subitem_text_value":"Nemoto, Naoto"},{"subitem_text_value":"Fukushima, Hideo"},{"subitem_text_value":"Nishigaki, Koichi"}]},"item_120_text_32":{"attribute_name":"アイテムID","attribute_value_mlt":[{"subitem_text_value":"KY-AA11808968-02-34"}]},"item_120_text_4":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"株式会社ジェンコム研究開発部"},{"subitem_text_value":"埼玉大学工学部機能材料工学科"},{"subitem_text_value":"埼玉大学工学部機能材料工学科"},{"subitem_text_value":"株式会社ジェンコム研究開発部"},{"subitem_text_value":"株式会社ジェンコム研究開発部"},{"subitem_text_value":"埼玉大学工学部機能材料工学科"}]},"item_120_text_5":{"attribute_name":"著者 所属(別言語)","attribute_value_mlt":[{"subitem_text_value":"Department of Research and Development, GenCom Co., Ltd."},{"subitem_text_value":"Department of Functional Materials Science, Saitama University"},{"subitem_text_value":"Department of Functional Materials Science, Saitama University"},{"subitem_text_value":"Department of Research and Development, GenCom Co., Ltd."},{"subitem_text_value":"Department of Research and Development, GenCom Co., Ltd."},{"subitem_text_value":"Department of Functional Materials Science, Saitama University"}]},"item_120_text_9":{"attribute_name":"年月次","attribute_value_mlt":[{"subitem_text_value":"2001"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 輝章"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"楢崎, 真介"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木下, 保則"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"根本, 直人"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"福島, 秀郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西垣, 功一"},{"creatorName":"ニシガキ, コウイチ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-24"}],"displaytype":"detail","filename":"KY-AA11808968-02-34.pdf","filesize":[{"value":"103.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KY-AA11808968-02-34.pdf","url":"https://sucra.repo.nii.ac.jp/record/16640/files/KY-AA11808968-02-34.pdf"},"version_id":"9f7bb576-b6aa-4633-85c2-3352dd216dce"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Y-ligation","subitem_subject_scheme":"Other"},{"subitem_subject":"block shuffling","subitem_subject_scheme":"Other"},{"subitem_subject":"evolutionary protein engineering","subitem_subject_scheme":"Other"},{"subitem_subject":"peptide","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"YLBSによるペプチドライブラリーの作成と利用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"YLBSによるペプチドライブラリーの作成と利用"}]},"item_type_id":"120","owner":"3","path":["634"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-06-24"},"publish_date":"2009-06-24","publish_status":"0","recid":"16640","relation_version_is_last":true,"title":["YLBSによるペプチドライブラリーの作成と利用"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-15T18:16:35.114429+00:00"}