@phdthesis{oai:sucra.repo.nii.ac.jp:00019688,
 author = {髙尾, 理沙},
 month = {},
 note = {60p, 第1章 序論
　　　第1節 序 ····················································································· 1

第2章 Kinanthraquinone (KQ) 生合成遺伝子クラスターの同定 ·························· 3
　　　第1節 KQ生合成遺伝子クラスターの探索 ········································· 3
　　　第2節 異種発現ベクターの構築及び形質転換株の解析 ························· 8
　　　第3節 kiqG遺伝子破壊株の解析 ······················································ 9
　　　第4節 非典型OmpRファミリー制御因子遺伝子 ································· 10
　　　第5節 kiqA遺伝子発現によるKQ類縁体の生産性改善 ························ 12
　　　第6節 KQ類縁体の構造決定 ·························································· 13
　　　第7節 Feeding実験によるKQBからKQへの変換 ······························ 16
　　　第8節 考察 ····················································································· 17

第3章 KQ生合成遺伝子クラスターの解析と新規化合物の生物活性評価 ············· 18
　　　第1節 遺伝子破壊と生合成中間体の解析 ·········································· 18
　　　第2節 kiqO遺伝子破壊株に蓄積した化合物の単離・構造決定 ·············· 20
　　　第3節 Feeding実験による6,9-DHTからKQへの変換 ······························ 23
　　　第4節 6,9-DHTの生物活性評価 ······················································· 24
　　　第5節 配糖化経路の探索 ······························································· 25
　　　第6節 考察 ················································································· 27

実験の部 ···································································································· 29
参考文献 ···································································································· 39
補遺 ·········································································································· 46
謝辞 ·········································································································· 59, 主指導教員 : 高橋俊二, text, application/pdf},
 school = {埼玉大学},
 title = {放線菌二次代謝産物kinanthraquinoneの生合成研究},
 year = {2021},
 yomi = {タカオ, リサ}
}