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Biochemical Properties of a cAMP Phosphodiesterase in the Cyanobacterium Anabaena sp. strain PCC 7120
https://sucra.repo.nii.ac.jp/records/14149
https://sucra.repo.nii.ac.jp/records/14149be9667b2-0909-4652-9bbf-8de22a318c4d
名前 / ファイル | ライセンス | アクション |
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A1003671.pdf (562.1 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-10-20 | |||||
タイトル | ||||||
タイトル | Biochemical Properties of a cAMP Phosphodiesterase in the Cyanobacterium Anabaena sp. strain PCC 7120 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cyclic nucleotide phosphodiesterase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cAMP signal transduction | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cyanobacteria | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
FUJISAWA, TAKATOMO
× FUJISAWA, TAKATOMO× OHMORI, MASAYUKI |
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著者 ローマ字 | ||||||
FUJISAWA, TAKATOMO | ||||||
著者 ローマ字 | ||||||
OHMORI, MASAYUKI | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
東邦大学 ; 埼玉大学理学部 | ||||||
著者 所属(別言語) | ||||||
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo | ||||||
著者 所属(別言語) | ||||||
Center of Systems Biology and Ecology, Faculty of Science, Toho University ; (Present address)Department of Biochemistry and Molecular Biology, Faculty of Science, Saitama University | ||||||
書誌情報 |
Microbes and environments 巻 20, 号 2, p. 92-96, 発行日 2005 |
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年月次 | ||||||
2005-6 | ||||||
出版者名 | ||||||
出版者 | 日本微生物生態学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13426311 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1264/jsme2.20.92 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A gene for cAMP phosphodiesterase, designated cpdA, was identified in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120. The predicted amino acid sequence of the gene was similar to the sequences of cAMP phosphodiesterases from Thermosynechococcus elongatus, Escherichia coli and Haemophilus influenzae. The recombinant protein was purified by sequential column chromatography and its biochemical properties were determined. The Anabaena cAMP phosphodiesterase hydrolyzed cAMP and cGMP with similar levels of activity. The K_m value for cAMP was 45μM and the Vmax was 4.9μmol min^<-1> mg protein^<-1>. These values are similar to those of Escherichia coli cAMP phosphodiesterase. The enzyme was activated by divalent cations such as Fe^<2+> and Mn^<2+>. The tertiary structure of this enzyme was predicted by homology modeling. The deduced structure has two metal-binding sites in the catalytic domain. | |||||
注記 | ||||||
内容記述タイプ | Other | |||||
内容記述 | rights: 日本微生物生態学会 rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである relation: IsVersionOf: http://ci.nii.ac.jp/naid/110001282480/ |
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版 | ||||||
[出版社版] | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2009-10-08 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1003671 |