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架橋構造を有する分子認識ペプチドの試験管内淘汰に関する研究
https://doi.org/10.24561/00010307
https://doi.org/10.24561/00010307fad8aeec-49e6-475e-b6b1-12d0c79a824d
名前 / ファイル | ライセンス | アクション |
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GD0000530.pdf (2.6 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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公開日 | 2015-02-04 | |||||||||
タイトル | ||||||||||
言語 | ja | |||||||||
タイトル | 架橋構造を有する分子認識ペプチドの試験管内淘汰に関する研究 | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
資源タイプ | doctoral thesis | |||||||||
ID登録 | ||||||||||
ID登録 | 10.24561/00010307 | |||||||||
ID登録タイプ | JaLC | |||||||||
アクセス権 | ||||||||||
アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
著者 |
望月, 佑樹
× 望月, 佑樹
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著者 所属 | ||||||||||
埼玉大学大学院理工学研究科(博士後期課程)理工学専攻 | ||||||||||
著者 所属(別言語) | ||||||||||
Graduate School of Science and Engineering, Saitama University | ||||||||||
書誌 | ||||||||||
収録物名 | 博士論文(埼玉大学大学院理工学研究科(博士後期課程)) | |||||||||
書誌情報 |
発行日 2014 |
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出版者名 | ||||||||||
出版者 | 埼玉大学大学院理工学研究科 | |||||||||
出版者名(別言語) | ||||||||||
出版者 | Graduate School of Science and Engineering, Saitama University | |||||||||
形態 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 69 p. | |||||||||
学位授与番号 | ||||||||||
学位授与番号 | 甲第940号 | |||||||||
学位授与年月日 | ||||||||||
学位授与年月日 | 2014-03-24 | |||||||||
学位名 | ||||||||||
学位名 | 博士(工学) | |||||||||
学位授与機関 | ||||||||||
学位授与機関識別子Scheme | kakenhi | |||||||||
学位授与機関識別子 | 12401 | |||||||||
学位授与機関名 | 埼玉大学 | |||||||||
目次 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | Chapter 1: Introduction 1 1-1. Peptide aptamer 1 1-2. Disulfide-rich peptides 4 1-3. In vitro display technologies 7 1-4. cDNA display 10 1-5. Aims of this study 14 Chapter 2: Improvement of in vitro selection/evolution system by cDNA display method 15 2-1. Increasing the library size in cDNA display by optimizing purification procedures 15 2-1-1. Introduction 15 2-1-2. Materials and methods 17 2-1-3. Results and discussion 18 2-2. A pull-down method with a biotinylated bait protein prepared by cell-free translation using a puromycin-linker. 24 2-2-1. Introduction 24 2-2-2. Materials and methods 25 2-2-3. Results and discussion 28 2-3. Conclusions 32 Chapter 3: In vitro selection of disulfide-rich peptides against amino groups by cDNA display method 33 3-1. Introduction 33 3-2. Materials and methods 35 3-2-1. Library construction 35 3-2-2. SBP-linker synthesis 37 3-2-3. cDNA display preparation from library DNAs 39 3-2-4. Affinity selection 41 3-2-5. Peptides 43 3-2-6. Trypsin digestion and mass spectrometry analysis 44 3-2-7. Binding assay against amino group modified magnetic beads 44 3-2-8. Binding assay against a glass slide 45 3-2-9. Binding assay against agarose beads 45 3-2-10. Circular dichroism analysis 46 3-3. Results and discussion 47 3-3-1. In vitro selection of amino group binding peptides 48 3-3-2. Binding assay by a pull-down method 49 3-3-3. Analysis of the relationship between disulfide connectivity and function 50 3-3-4. Binding assay against amino groups on several kinds of solid supports 54 3-3-5. Binding assay of CP1 (2SS)-α derivative peptides 55 3-3-6. Secondary structure analysis 57 3-4. Conclusions 59 Chapter 4: Overall conclusions 60 References 61 Acknowledgments 69 |
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注記 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 主指導教員 : 根本直人 | |||||||||
版 | ||||||||||
[出版社版] | ||||||||||
著者版フラグ | ||||||||||
出版タイプ | VoR | |||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
資源タイプ | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | text | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
作成日 | ||||||||||
日付 | 2015-02-04 | |||||||||
日付タイプ | Created | |||||||||
アイテムID | ||||||||||
GD0000530 |