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  1. 理工学研究科
  2. 01 学術雑誌論文

Activation of the rcs signal transduction system is responsible for the thermosensitive growth defect of an Escherichia coli mutant lacking phosphatidylglycerol and cardiolipin

https://sucra.repo.nii.ac.jp/records/12484
https://sucra.repo.nii.ac.jp/records/12484
20d14ec1-ba32-4a15-812a-024bd5a1e7ea
名前 / ファイル ライセンス アクション
A1002021.pdf A1002021.pdf (1.2 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2007-03-08
タイトル
タイトル Activation of the rcs signal transduction system is responsible for the thermosensitive growth defect of an Escherichia coli mutant lacking phosphatidylglycerol and cardiolipin
言語
言語 jpn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 原, 弘志

× 原, 弘志

WEKO 19550

原, 弘志

ja-Kana ハラ, ヒロシ

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著者 ローマ字
Hara, Hiroshi
著者 ローマ字
Shiba, Yasuhiro
著者 ローマ字
Yokoyama, Yasuko
著者 ローマ字
Aono, Yoshiko
著者 ローマ字
Kiuchi, Takashi
著者 ローマ字
Kusaka, Jin
著者 ローマ字
Matsumoto, Kouji
著者 所属
埼玉大学大学院理工学研究科
著者 所属(別言語)
Saitama University, Graduate School of Science and Engineering
書誌情報 Journal of bacteriology

巻 186, 号 19, 発行日 2004
年月次
2004
出版者名
出版者 American Society for Microbiology
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1128/JB.186.19.6526-6535.2004
抄録
内容記述タイプ Abstract
内容記述 Lethal effect of the Escherichia coli pgsA null mutation which causes a complete lack of the major acidic phospholipids, phosphatidylglycerol and cardiolipin, is alleviated by the lack of the major outer membrane lipoprotein encoded by the lpp gene, but the lpp pgsA strain shows a thermosensitive growth defect. By transposon mutagenesis we found that the hermosensitivity was suppressed by disruption of the rcsC, rcsF, and yojN genes that code for sensor kinase, accessory positive factor, and phosphotransmitter, respectively, of the Rcs phosphorelay signal transduction system initially identified as regulating the capsular polysaccharide synthesis genes (cps). Disruption of the rcsB gene coding for the response regulator of the system also suppressed the thermosensitivity, whereas disruption of cpsE did not. By monitoring the expression of a cpsB′-lac fusion we showed the Rcs system was activated in the pgsA mutant and reversed to a wild-type level by the rcs mutations. These results indicate that the envelope stress due to acidic phospholipid deficiency activates the Rcs phosphorelay system and thereby causes the thermosensitive growth defect independently of the activation of capsule synthesis.
資源タイプ
内容記述タイプ Other
内容記述 text
フォーマット
内容記述タイプ Other
内容記述 application/pdf
作成日
日付 2007-03-05
日付タイプ Created
アイテムID
A1002021
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