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Regulation of Translation by the Redox State of Elongation Factor G in the Cyanobacterium Synechocystis sp PCC 6803
https://sucra.repo.nii.ac.jp/records/13779
https://sucra.repo.nii.ac.jp/records/1377987a5ee5a-5d22-43ed-a4df-19dad1559a7d
名前 / ファイル | ライセンス | アクション |
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A1002932.pdf (1.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-08-20 | |||||
タイトル | ||||||
タイトル | Regulation of Translation by the Redox State of Elongation Factor G in the Cyanobacterium Synechocystis sp PCC 6803 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kojima, Kouji
× Kojima, Kouji× Motohashi, Ken× Morota, Takuya× Oshita, Masaru× Hisabori, Toru× Hayashi, Hidenori× 西山, 佳孝 |
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著者 ローマ字 | ||||||
Kojima, Kouji | ||||||
著者 ローマ字 | ||||||
Motohashi, Ken | ||||||
著者 ローマ字 | ||||||
Morota, Takuya | ||||||
著者 ローマ字 | ||||||
Oshita, Masaru | ||||||
著者 ローマ字 | ||||||
Hisabori, Toru | ||||||
著者 ローマ字 | ||||||
Hayashi, Hidenori | ||||||
著者 ローマ字 | ||||||
Nishiyama, Yoshitaka | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
著者 所属 | ||||||
埼玉大学大学院理工学研究科生命科学部門 | ||||||
著者 所属(別言語) | ||||||
Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, Saitama University | ||||||
著者 所属(別言語) | ||||||
Chemical Resources Laboratory, Tokyo Institute of Technology | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
著者 所属(別言語) | ||||||
Department of Biochemistry and Molecular Biology, Graduate School of Science | ||||||
書誌情報 |
JOURNAL OF BIOLOGICAL CHEMISTRY 巻 284, 号 28, p. 18685-18691, 発行日 2009 |
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年月次 | ||||||
2009-7 | ||||||
出版者名 | ||||||
出版者 | American Society for Biochemistry and Molecular Biology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00219258 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1074/jbc.M109.015131 | |||||
関連サイト | ||||||
内容記述タイプ | Other | |||||
内容記述 | http://www.jbc.org/cgi/content/abstract/284/28/18685 | http://www.jbc.org/cgi/content/abstract/284/28/18685 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Elongation factor G (EF-G), a key protein in translational elongation, was identified as a primary target of inactivation by reactive oxygen species within the translational machinery of the cyanobacterium Synechocystis sp. PCC 6803 (Kojima, K., Oshita, M., Nanjo, Y., Kasai, K., Tozawa, Y., Hayashi, H., and Nishiyama, Y. (2007) Mol. Microbiol. 65, 936–947). In the present study, we found that inactivation of EF-G (Slr1463) byH2O2 was attributable to the oxidation of two specific cysteine residues and formation of a disulfide bond. Substitution of these cysteine residues by serine residues protected EF-G from inactivation byH2O2 and allowed the EF-G to mediate translation in a translation system in vitro that had been prepared from Synechocystis. The disulfide bond in oxidized EF-G was reduced by thioredoxin, and the resultant reduced form of EF-G regained the activity to mediate translation in vitro. Western blotting analysis showed that levels of the oxidized form of EF-G increased under strong light in a mutant that lacked NADPHthioredoxin reductase, indicating that EF-G is reduced by thioredoxin in vivo. These observations suggest that the translational machinery is regulated by the redox state of EF-G, which is oxidized by reactive oxygen species and reduced by thioredoxin, a transmitter of reducing signals generated by the photosynthetic transport of electrons. |
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[出版社版] | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
資源タイプ | ||||||
内容記述タイプ | Other | |||||
内容記述 | text | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
作成日 | ||||||
日付 | 2010-08-20 | |||||
日付タイプ | Created | |||||
アイテムID | ||||||
A1002932 |